E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati.

Abstract
To diagnose malaria, microscopic examination is still considered as the standard tool, but still there are certain reservations. This study aimed to compare the identification of Plasmodium species by microscopy and polymerase chain reaction (PCR). This cross-sectional investigation was carried out in June 2019 until September 2019. Blood samples were taken and examined using microscope and nested PCR. Both results were compared to evaluate the diagnostic performance of microscopy and agreement between both examinations. Only P. vivax and P. falciparum were found in current study. The sensitivity, specificity, PPV, and NPV of the microscopy examination compared to nested PCR for detecting P. vivax, was 91.0%, 97.5%, 96.8%, and 92.8% respectively with κ=0.889; and for P. falciparum, the sensitivity, specificity, PPV and NPV was 62.7%, 95.8%, 88.9%, and 82.7% respectively with κ=0.628. Among all mixed infections, only 12% were identified correctly by microscopy. Microscopy is reliable way for routine diagnostic purposes, to detect single malaria infection. However, it is unreliable to detect mixed malaria infections.

Key words: Malaria, microscopy, polymerase chain reaction.


 
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How to Cite this Article
Pubmed Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. SRP. 2020; 11(6): 91-95. doi:10.31838/srp.2020.6.16


Web Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. http://www.sysrevpharm.org//?mno=112580 [Access: June 05, 2020]. doi:10.31838/srp.2020.6.16


AMA (American Medical Association) Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. SRP. 2020; 11(6): 91-95. doi:10.31838/srp.2020.6.16



Vancouver/ICMJE Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. SRP. (2020), [cited June 05, 2020]; 11(6): 91-95. doi:10.31838/srp.2020.6.16



Harvard Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati (2020) Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. SRP, 11 (6), 91-95. doi:10.31838/srp.2020.6.16



Turabian Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. 2020. Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. Systematic Reviews in Pharmacy, 11 (6), 91-95. doi:10.31838/srp.2020.6.16



Chicago Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. "Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia." Systematic Reviews in Pharmacy 11 (2020), 91-95. doi:10.31838/srp.2020.6.16



MLA (The Modern Language Association) Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati. "Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia." Systematic Reviews in Pharmacy 11.6 (2020), 91-95. Print. doi:10.31838/srp.2020.6.16



APA (American Psychological Association) Style

Christophorus Oetama Adiatmaja, Trieva Verawaty Butarbutar, Amarensi Milka Betaubun, Puspa Wardhani, Aryati (2020) Comparison of Malaria Microscopy and Polymerase Chain Reaction for Identification of Plasmodium in Papua, Indonesia. Systematic Reviews in Pharmacy, 11 (6), 91-95. doi:10.31838/srp.2020.6.16





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