E-ISSN 0976-2779 | ISSN 0975-8453
 

Research Article 


Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice

Saikhu Akhmad Husen, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, Win Darmanto.

Abstract
Introduction: Diabetes mellitus (DM) is a chronic disease with diverse and complex metabolic disorders. DM can be identified with hyperglycemic conditions. In 2017, Indonesia ranked sixth in the world for the highest number of diabetic patients after China, India, United States, Brazil, and Mexico.
Objective: This study aims to determine the combined effect of alginate extract from Sargassum duplicatum and Sargassum ilicifolium with okra (Abelmoschus esculentus) and mangosteen (Garcinia mangostana) pericarp extracts to ameliorate the wound width process, the number of neutrophil cells, macrophages, fibroblasts, fibrocytes, and collagen density in streptozotocin-induced diabetic mice.
Methods: This study used male mice (strain BALB/C, weighting 30±5 g each) which were grouped into four groups; normal control with water only; diabetic control group; combination treatment group with S. duplicatum extract, A. esculentus extract, and G. mangostana extract; and combination treatment group with S. ilicifolium extract, A. esculentus extract, and G. mangostana extract. The diabetic control group and combination treatment groups were injected with multiple low doses of streptozotocin (STZ) at 30 mg/kg BW intraperitoneally. The combination extract dose given in treatment groups was 50 mg/kg BW. Measurement of fasting blood sugar levels was done before and after the STZ injection. The mice’s skin was cut in the gluteal section as long as 1 cm, then treated topically in three different time periods namely 3, 7, and 14 days. Histological preparation and analysis were conducted on the 15th day.
Results: The administration of A. esculentus extract and G. mangostana extract combined with S. duplicatum and S. ilicifolium increased wound healing indicator rate, decreased neutrophile cell count (NCC) and fibrocyte cell count (FCCC), and increased collagen synthesis as an extracellular matrix (ECM). The treatments could restore the open wound healing process of diabetic mice to their normal state. There were no significant differences in macrophage cell count (MCC) and fibroblast cell count (FBCC). The histopathology showed that the combination treatment groups also could accelerate the wound healing process in mice skin.
Conclusion: In summary, this study addressed that the administration of A. esculentus extract and G. mangostana extract combined with S. duplicatum and S. silicifolium extracts could ameliorate open wound healing in diabetic mice.

Key words: Sargassum, Abelmoschus esculentus, Garcinia mangostana, open wound healing, diabetic mice


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Saikhu Akhmad Husen
Articles by Muhammad Farraz Syadzha
Articles by Muhamad Frendy Setyawan
Articles by Pratiwi Pudjiastuti
Articles by Arif Nur Muhammad Ansori
Articles by Raden Joko Kuncoroningrat Susilo
Articles by Suhailah Hayaza
Articles by Dwi Winarni
Articles by Win Darmanto
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP. 2020; 11(9): 888-892. doi:10.31838/srp.2020.9.129


Web Style

Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. http://www.sysrevpharm.org/?mno=22602 [Access: December 02, 2020]. doi:10.31838/srp.2020.9.129


AMA (American Medical Association) Style

Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP. 2020; 11(9): 888-892. doi:10.31838/srp.2020.9.129



Vancouver/ICMJE Style

Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP. (2020), [cited December 02, 2020]; 11(9): 888-892. doi:10.31838/srp.2020.9.129



Harvard Style

Husen, S. A., Syadzha, . M. F., Setyawan, . M. F., Pudjiastuti, . P., Ansori, . A. N. M., Susilo, . R. J. K., Hayaza, . S., Winarni, . D. & Darmanto, . W. (2020) Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP, 11 (9), 888-892. doi:10.31838/srp.2020.9.129



Turabian Style

Husen, Saikhu Akhmad, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, and Win Darmanto. 2020. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. Systematic Reviews in Pharmacy, 11 (9), 888-892. doi:10.31838/srp.2020.9.129



Chicago Style

Husen, Saikhu Akhmad, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, and Win Darmanto. "Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice." Systematic Reviews in Pharmacy 11 (2020), 888-892. doi:10.31838/srp.2020.9.129



MLA (The Modern Language Association) Style

Husen, Saikhu Akhmad, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, and Win Darmanto. "Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice." Systematic Reviews in Pharmacy 11.9 (2020), 888-892. Print. doi:10.31838/srp.2020.9.129



APA (American Psychological Association) Style

Husen, S. A., Syadzha, . M. F., Setyawan, . M. F., Pudjiastuti, . P., Ansori, . A. N. M., Susilo, . R. J. K., Hayaza, . S., Winarni, . D. & Darmanto, . W. (2020) Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. Systematic Reviews in Pharmacy, 11 (9), 888-892. doi:10.31838/srp.2020.9.129





Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context
    Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran
    SRP. 2020; 11(1): 14-22
    » Abstract » doi: 10.5530/srp.2020.1.03

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer
    Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova
    SRP. 2019; 10(1): 112-117
    » Abstract » doi: 10.5530/srp.2019.1.19

  • Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review
    Karle Pravin P, Dhawale Shashikant C
    SRP. 2019; 10(1): 11-14
    » Abstract » doi: 0.5530/srp.2019.1.2

  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba
    Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar
    SRP. 2019; 10(1): 20-25
    » Abstract » doi: 10.5530/srp.2019.1.4

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • Most Cited Articles