Abstract
Background and objective: In recent years, vitamin D has been considered an interesting subject of study due to its pleiotropic role including autocrine, paracrine and endocrine function in several target organs and systems. The essential molecule for reproduction in animals and it have an antioxidant properties in all mammalian cells is known vitamin E. This study aimed the study the effects of acute supplementation of both vitamins D and E on developing of testosterone levels and reproductive properties in rats.
Methods: Forty rats were divided into four groups, 1st group were feeding by acute supplementation with vitamin D while 2nd group supplementation of vitamin E, 3rd group feeding with acute supplementation of both vitamins, and 4th group was control with normal fed. HPLC technique was used to assessing of vitamins D and E levels while levels of TES were investigated by ELISA.
Results: The levels of vitamins D (ng/ml) levels in this experiment (after month of supplementation) of study groups (VDS, VES, VDES, and Control) were 7.27±2.1 ,3.15±1.1, 6.19±0.9, 5.11±1.4, respectively, while vitamin E (ng/ml) levels were 4.13±1.2, 6.4±1.3 ,3.15±1.1, 4.17±1.3, respectively, and the p-value was < 0.05 when comparing between all groups. TES levels (pg/ml) were 13.40±3.9, 16.04±2.6, 17.18±3.5, 11.17±2.7 in study groups and the p-value also <0.05 and significant statistical.
Conclusion: Combination of both vitamins D and E as acute supplementation will increase TES levels more than vitamin D or E alone in male rats.

Key words: Vitamin D, vitamins E, Acute supplementation, Testosterone, Rats