E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer

Basma Talib Al-Sudani, Baydaa Hameed, Ahmed T Alahmar.

Abstract
Among age-related morality in humans, cancer stands at 2nd leading cause and this is why the ability to suppress carcinogenesis has got the cancer prevention and treatment researchers working hard on this. Leukemia (cancer of bone marrow and blood), is most commonly characterized by uncontrolled proliferation of some blood cells, in this case leukocytes. There are generally four types of leukemia: acute myelogenous, chronic myelogenous, acute lymphocytic and chronic myelogenous. There are quite a few different leukemia cell lines and among them, the MOTN-1 cell line was derived from acute lymphoblastic leukemia. Moreover, the MOTN-1 cells are commonly described a highly undifferentiated leukemia cells. In this study, we evaluated the effects of SIRT1 activation by aptamer (Aptamer are single strand DNA or RNA molecules, selected by an iterative process known as Systematic Evolution of Ligands by Exponential Enrichment SELEX) on viability of MOTN-1 cancer cell line as an anticancer drug and analysed the gene expression profiles of MOTN-1 cells treated with aptamer and untreated cells by cDNA microarray analysis and RT-PCR to give us more details about the molecular mechanisms underlying aptamer- induced carcinogenesis, including leukaemia. The results demonstrate that aptamer is extremely efficient tools for anticancer activity on ALL by inhibited 91% of these cancer cells at 10 µM aptamer after 72 hours with IC50 = 3.5 ÎĽM. Besides that, we at first identified 13 genes with expression changed by aptamer treatment. For this set of genes, 11 of these were up-regulated and two were down-regulated by more than 3-fold, respectively. As a result, pharmacological activation of SIRT1 enhanced cell death suggesting a tumor suppressive function of aptamer and may be used in the future for cancer treatment.

Key words: Microarray, Aptamer, Cytotoxicity, Gene expression, Acute Lymphoblastic Leukaemia.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Basma Talib Al-Sudani
Articles by Baydaa Hameed
Articles by Ahmed T Alahmar
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Al-Sudani BT, Hameed B, Alahmar AT. Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. SRP. 2020; 11(2): 123-134. doi:10.5530/srp.2020.2.20


Web Style

Al-Sudani BT, Hameed B, Alahmar AT. Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. http://www.sysrevpharm.org//?mno=87628 [Access: June 01, 2020]. doi:10.5530/srp.2020.2.20


AMA (American Medical Association) Style

Al-Sudani BT, Hameed B, Alahmar AT. Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. SRP. 2020; 11(2): 123-134. doi:10.5530/srp.2020.2.20



Vancouver/ICMJE Style

Al-Sudani BT, Hameed B, Alahmar AT. Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. SRP. (2020), [cited June 01, 2020]; 11(2): 123-134. doi:10.5530/srp.2020.2.20



Harvard Style

Al-Sudani, B. T., Hameed, . B. & Alahmar, . A. T. (2020) Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. SRP, 11 (2), 123-134. doi:10.5530/srp.2020.2.20



Turabian Style

Al-Sudani, Basma Talib, Baydaa Hameed, and Ahmed T Alahmar. 2020. Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. Systematic Reviews in Pharmacy, 11 (2), 123-134. doi:10.5530/srp.2020.2.20



Chicago Style

Al-Sudani, Basma Talib, Baydaa Hameed, and Ahmed T Alahmar. "Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer." Systematic Reviews in Pharmacy 11 (2020), 123-134. doi:10.5530/srp.2020.2.20



MLA (The Modern Language Association) Style

Al-Sudani, Basma Talib, Baydaa Hameed, and Ahmed T Alahmar. "Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer." Systematic Reviews in Pharmacy 11.2 (2020), 123-134. Print. doi:10.5530/srp.2020.2.20



APA (American Psychological Association) Style

Al-Sudani, B. T., Hameed, . B. & Alahmar, . A. T. (2020) Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. Systematic Reviews in Pharmacy, 11 (2), 123-134. doi:10.5530/srp.2020.2.20





Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context
    Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran
    SRP. 2020; 11(1): 14-22
    » Abstract » doi: 10.5530/srp.2020.1.03

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer
    Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova
    SRP. 2019; 10(1): 112-117
    » Abstract » doi: 10.5530/srp.2019.1.19

  • Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study
    Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli
    SRP. 2020; 11(1): 01-06
    » Abstract » doi: 10.5530/srp.2020.1.01

  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review
    Karle Pravin P, Dhawale Shashikant C
    SRP. 2019; 10(1): 11-14
    » Abstract » doi: 0.5530/srp.2019.1.2

  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba
    Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar
    SRP. 2019; 10(1): 20-25
    » Abstract » doi: 10.5530/srp.2019.1.4

  • A Review of Pharmacoeconomics: the key to “Healthcare for All”
    Hasamnis AA, Patil SS, Shaik Imam, Narendiran K
    SRP. 2019; 10(1): s40-s42
    » Abstract » doi: 10.5530/srp.2019.1s.21

  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata
    Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi
    SRP. 2019; 10(1): 15-19
    » Abstract » doi: 10.5530/srp.2019.1.3

  • Most Cited Articles