E-ISSN 0976-2779 | ISSN 0975-8453
 

Review Article 


Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed.

Abstract
A simple ZIC-HILIC-UV technique was developed and applied for the determination of 2-deoxyguanosine in the human serum. The separa-tion took place in the ZIC1 and ZIC5 self-designed stationary phases, in gradient elution mode with a buffer for acetonitrile acetate and 254 nm UV detection. The 2-deoxyguanosine conduct with varying acetate buffer, ACN and pH values has been tested and the results have established the hydrophilicity of 2-deoxyguanosine. The mechanism of separation is based on the hydrophilic and ion exchange partitioning of the2-deoxyguanosine. We have defined and mentioned the current effect of both ZIC1 and ZIC5 stationary stationary phenomena on chromatographic conditions (sodium acetate buffer concentration, ACN and pH). All existing methods are a useful alternative to the current 2-deoxyguanosine separation methods.

Key words: 2-deoxyguanosine, cation exchange, nucleoside, HILIC stationary phase, hydrophilic interaction


 
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How to Cite this Article
Pubmed Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP. 2020; 11(3): 240-246. doi:10.5530/srp.2020.3.27


Web Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. http://www.sysrevpharm.org//?mno=92890 [Access: April 08, 2020]. doi:10.5530/srp.2020.3.27


AMA (American Medical Association) Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP. 2020; 11(3): 240-246. doi:10.5530/srp.2020.3.27



Vancouver/ICMJE Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP. (2020), [cited April 08, 2020]; 11(3): 240-246. doi:10.5530/srp.2020.3.27



Harvard Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed (2020) Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. SRP, 11 (3), 240-246. doi:10.5530/srp.2020.3.27



Turabian Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. 2020. Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. Systematic Reviews in Pharmacy, 11 (3), 240-246. doi:10.5530/srp.2020.3.27



Chicago Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. "Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum." Systematic Reviews in Pharmacy 11 (2020), 240-246. doi:10.5530/srp.2020.3.27



MLA (The Modern Language Association) Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed. "Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum." Systematic Reviews in Pharmacy 11.3 (2020), 240-246. Print. doi:10.5530/srp.2020.3.27



APA (American Psychological Association) Style

Yaqout Abd Al-Hakeem Hamed, Ashraf Saad Rasheed (2020) Zwitterionic Ion Chromatography Coupled with Ultra-violet Detection for the Quantification of 2-Deoxyguanosine in Human Serum. Systematic Reviews in Pharmacy, 11 (3), 240-246. doi:10.5530/srp.2020.3.27





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