Analytical Techniques Used in Metabolomics: A Review
Metabolomics is a branch of chemistry in which endogenous metabolites present in a biological sample are accessed and efforts are made for their identification as well as quantification. The emerging scope and vast plant metabolite profiling has resulted in the development and advancement of modern plant metabolomics and it came out to be an extremely significant tool for attaining a thorough knowledge related to plant biology and physiology. To successfully identify and quantify the plant metabolites, there is a need of compatible as well as reliable analytical techniques. In the recent years, modern analytical techniques like NMR, GC-MS/MS, CE-MS/MS, LC-MS/MS etc. have proved to be quite efficient in the field of metabolomics for characterization and quantification purpose. The present review provides an insight to the utility and applications of the commonly used analytical techniques along with their advantages and disadvantages in the field of metabolomics.