Method Development and Validation of Labetalol and Nadolol in Human Plasma by Lc-Ms/Ms
Pagidi Rajagopaludu*, Nimmakayala Saritha, Devanna N, Srinivas M
LC-MS/MS technique is used for method development and validation of labetalol and nadolol in human plasma. Liquid chromatography separates mixture components in the basis of differences in affinity for stationary and mobile phase. It removes undesired impurities. It increases reproducibility, sensitivity, robustness, detection of low-level proteins. C18 Column (Phenomenex Luna C18, 5 μm, 50*4.6 mm ID) is used to for high resolution and peak area. Calibration curve is constructed with the help of linear regression. During the entire process no instability is found. The mass to charge ratio of labetalol and nadolol is 329.3→162 and 310.2→254.2. The average recovery of Labetalol is 91.3% and Nadolol is 100.6%.