Antibacterial and Phytochemical Analysis of Two Plants Menispermaceous Family

Abstract

Silvia Anitasari, Sjarif Ismail, Bayu Satria Wiratama, Hendrik Setia Budi

The failure of antibiotic treatment due to resistant bacteria made emerging cases in this century. This situation needs new antimicrobial from nature to cure it. Coscinium fenestratum (CF) and Fibraurea tinctoria (FT), both plants from Borneo usually were used by local ethnic to treatment various diseases caused bacteria. Hence, our study to determine antibacterial activity of CF and FT stem and finding secondary metabolite which could inhibit or kill bacteria. This study used extract ethanol of the stem plants, MSSA (ATCC 43300), MRSA (ATCC 25923), and local isolates of MDR E. coli. Antibacterial activity was measured by Kirby-Bauer Method, MICs and MBCs, while TLC for secondary metabolites Value of inhibition zone for extract of CF based on concentration was MSSA (8.1±0.1), MRSA (8.1±0.0), MDR E.coli (8.1±0.1) to 1mg/ml; MSSA (8.1±0.0), MRSA (10.0±0.1), MDR E.coli (8.1±0.0) to 2 mg/ml; MSSA(10.1±0.0), MRSA (12.1±0.0), MDR E.coli (10.0±0.0) to 4 mg/ml; MSSA (12.1±0.0), MRSA (12.1±0.0), MDR E. coli (12.0±0.0) to 8 mg/ml. While for FT was MSSA (8.1±0.0),MRSA (8.0±0.0), MDR E.coli (8.0±0.0) to 1 mg/ml; MSSA (8.0±0.0), MRSA (10.0±0.1), MDR E.coli (8.0±0.0) to 2 mg/ml; MSSA, MRSA, MDR E.coli to 4 mg/ml; MSSA, MRSA, MDR E.coli to 8 mg/ml. Meanwhile, MIC and MBC results for extract of CF was MIC (25 mg/ml), MBC (50 mg/ml) to MSSA; MIC (6.25 mg/ml), MBC (12.5 mg/ml) to MRSA; MIC (25 mg/ml), MBC (25 mg/ml) to MDR E. coli; while for FT was MIC (25 mg/ml), MBC (25 mg/ml) to MSSA; MIC (3.13 mg/ml), MBC (6.25 mg/ml) to MRSA; MIC (25 mg/ml), MBC ( 25 mg/ml) to MDR E.coli and then both extracts had retention factor 0.32 and 0.42 for secondary metabolites. Both plants had antibacterial effect but FT had stronger effect than another; therefore, it could be candidate for new antimicrobial.