ANTIDIABETIC POTENTIAL OF MATOA BARK EXTRACT (Pometia pinnata) IN ALLOXAN-INDUCED DIABETIC MALE RAT STRAIN WISTAR (Rattus norvegicus)

Abstract

Gita Sekar Prihanti, Raden Mochammad Kelvin Katjasungkana, Bella Ruli Novitasari, Shania Rizky Amalia, Audina Nurfajriana, Sulistyo Mulyo Agustini, Hanna Cakrawati, Desy Andari

Diabetesmellitusischaracterizedbyahyperglycemia,characterizedbyincreasedbloodsugarlevelandglycosylatedhemoglobin(HbA1c).HyperglycemiaalsoproducedexcessiveamountofReactiveOxygenSpecies(ROS)whichcausedincreasedlevelofserumMalondialdehyde(MDA)andprogressivechangesinthemeanamountofthepancreaticislets.MatoabarkextractisusedasanantidiabeticagentcontainingpolyphenolsandflavonoidswhichactsasantioxidantsagaintsReactiveOxygenSpecies(ROS)andalsoasanα-glucosidaseenzymeinhibitors.Todeterminetheantidiabeticeffectofmatoastembarkextractonalloxan-inducedWistarrats.Experimentalresearchdesignwithpost-testonlycontrolgroupdesign.Thecontrolgroupwaspositive,negative,and4treatmentgroupsconsistedof5experimentalanimalsineachgroupfor38days.BloodsugarandMDAaremeasuredusingaspectrophotometer.HbA1cwasmeasuredusingtheHPLCmethod.LangerhansIslandwasdyedHEcheckedusingamicroscope.DatawereanalyzedusingANOVA,PostHoc,andRegressiontests.ANOVAtestresultshaveasignificanteffect(p=0,000).PostHoctestresultsobtainedasignificantdifference(p<0.05)inalltreatmentgroups.MDAlevelsAtanewdoseof300mg/200gBW,therewasasignificantdifference(p<0.005).Matoabark89%todecreasebloodsugar,96.5%todecreaseinHbA1c,72%todecreaseMDA,82%totheaveragepictureofLangerhansIsland.Dosesthathaveasignificanteffecthavebeguntoappearatadoseof300mg/200gBWtothehighestdoseof400mg/200gBW.Matoastembarkextracthasbeenshowntohaveantidiabeticeffectsthataffectbloodsugarlevels,HbA1c,MDA,andthemeanofLangerhansIslandallistan-inducedmaleWistarrats(Rattusnorvegicus)