APOPTOSIS INDUCING POTENTIAL AND IMMUNOMODULATION OF COLOCASIA ESCULENTA AGAINST MYCOBACTERIAL SPECIES
Saeed Arrak Turkey,Mustafa M.Farhan, Essa D Alhtheal, kamal jawad awni.
Colocasia esculenta (C. esculenta) is said to be widely cultivated plant species of Araceae family, used for consumption as food and also used in medicine. of both leaves and tubers. Commonly called as Taro, and contains a vast range of bioactive compounds like flavanoids, terpenoids and alkaloids. Corms of this plant is said to processes pharmacological activities to treat several diseases. The present study is aimed to study the possible immunomodulatory and apoptotic potential of this sample to treat and cure against the
Mycobacteria species. The corms dried and extracted with methanol, hexane, acetone and dichloromethane and used to study the antimycobacterial activity by MIC activity and total activity. Its potent antiinflammatory activity was studied by the cytokine assay targeting the IL-6, TNF and Il-10 and apoptotic inducing potential by Annexin assay. The methanol extract showed maximum MIC values as compared to the positive control rifampicin. In accordance to the above result, methanol extract also showed an inducing apoptotic potential of 73±0.92 at 290Î¼g/ml (Rifampicin 89±2.12). Methanol extract also significantly reduced the IL-6 and TNF levels to 11.23 ± 0.23pg/ml and 9.29± 0.41 respectively at 290Î¼g/ml. It also enhanced the levels of IL-10 from 12.11± 0.71 (0.29Î¼g/ml) to
48.76± 0.81 (290Î¼g/ml); P < 0. 005.The present study confirmed the positive effect of the methanol extract as immunomodulatory and antiinflamatory by showing an inhibition in the expression of IL-6 and TNF. Thus in conclusion C.esculenta could be used as the best antiinflammatory candidate and immunomodulatory supplement due to its containing many bioactive compounds.