Association between Glycated Hemoglobin A1c Levels with Genomic Instability in Type 2 Diabetes Mellitus Patients

Abstract

Huda Muhammed Muzher, Farha A. Ali Shafi, Abdul Ameer N. Ghloub Alrekabi.

The incidence of type 2 diabetes mellitus globally increased. Genomic damage induced by oxidative stress play important role in the pathogenesis of diabetic complications. The objectives of this research are to determine whether increased blood glucose level in diabetes patients has been related with elevated levels of oxidation and DNA damage. Case –control study of (90) (Patients previously diagnosed with type 2 diabetes and 30 controls (non-diabetic apparently healthy volunteers) all participants were tested for plasma glycosylated hemoglobin (HbA1C), the DNA oxidation and genomic instability was performed by quantified of the level of 8-OHdG in urine samples and micronuclei and binucleated cells in exfoliated buccal cells respectively. The 8-OHdG contents in urine samples of diabetic patients were substantially high. However, there were non-significant differences within groups (P>0.05) The differences between8-OHdG contents in patients versus the control group were statistically significant (P<0.01) frequency of binucletied cells positive liner correlation with frequency of micronuclei strong positive correlation between frequency of micronuclei and HbA1C value. Our study points that micronuclei assay is a very valuable and noninvasive method predict genomic instability and its consequences mostly cancer in diabetes patients. Hence, clinical applications of MN assay may potentially improve the quality of administration of patients with diabetes and its complications.