Bioactivity Guided Isolation, Characterization & Pharmacological Evaluation of α-amyrin from Chloroform Extract of Morinda pubescens in STZ induced Diabetic Rats
Oday Sajjad Alsawad, Zainab A. Al-Mayyahi, Nameer A. Khudhair, Laith Hossein Jawid
AIM:This research has been designed for isolation of Î±-amyrin by chloroform extraction from Morindapubescens through characterization and pharmacological evaluation for this extraction and its effect on specific biochemical factors in STZ induced diabetic rats.
MATERIAL & METHODS-For the isolation of compounds, chloroform and methanol was used as a solvent system. Firstly 100% chloroform was passed through column and then increasing quantity of methanol was used in different ratio (90:10, 80:20, 70:30, 60:40 and finally 50:50). All the fractions were evaluated for antidiabetic activity (data not shown here) and fraction 3 showed a single spot in TLC and it was characterized by spectral techniques.
RESULTS-The compound was determined to be Î±-amyrin. The anti-diabetic activity with various biochemical markers was accessed in STZ induced diabetic rats. Plasma glucose levels increased significantly (P<0.01) in diabetic ratsother than the normal rats. Î±-amyrin was extremely meaningful (p<0.001) and marginally important (p<0.01) in lipid profile relative to diabetic group at dose ranges of 5 mg/kg and 10 m/kg. The effect of Î±-amyrin on different lipids as well as raised HDL levels relative to diabetic animals was highly important. The effect of Î±-amyrin at 5mg/kg and 10mg/kg in contrast to diabetic groups was extremely important (p<0.001) and mediallyimportant (p<0.01) in SGPT and SGOT.
CONCLUSION: Î±-amyrin extraction by chloroform from Morindapubescens plant was found to haveantilipidemic and antidiabetics effect through reduce the deleterious effect of diabetes in laboratory rat.