Comparative molecular study between two species of flour beetle Tribolium castaneum and Tribolium confusum by using RAPD-PCR

Abstract

Ammar Ahmed Sultan

This research was carried out for molecular comparison between two species of flour beetle T. castaneum and T. confusum in Iraq by using RAPD-PCR technique, because the difficulty of distinguishing between the two types in morphology, In this research used seven primers and genetic population analysis programs for calculates the percentage efficiency and discriminatory ability for each primer , As well as it is used genetic identity for Nei’s. The results obtained show that the primers Number 3 , 4 and 6 were given the highest number of amplified polymorphic fragments and the highest percentage of efficiency and the ability of discriminatory it is 7, 7, 20 and 21.2 on the sequentially ., While primer number 2 gave the lowest number of amplified polymorphic fragments and the lowest percentage of efficiency and the ability of discriminatory it is 2 , 2 , 5.71 and 6.06 on the sequentially ., When comparing between two types T. castaneum and T. confusum based on genetic identity it was observed that T. castaneum close genetically to T. confusum, the degree of genetic convergence is 0.111.