Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method
Abstract
Hani Plumeriastuti, Agesti Veva Kalista, Budiastuti; Mustofa Helmi Effendi.
The aim of this study was to identify the cytotoxicity effect of Tithonia diversifolia leaf extract on bone marrow mesenchymal stem cell using MTT assay. The sample of this research using bone marrow mesenchymal stem cell of rats. The Tithonia diversifolia leaf extract divide into 4 concentration (0.5%, 0.25%, 0.125%, and 0.0625%) and given to bone marrow mesenchymal stem cell of rats in each well and incubated for 24 hours. The toxicity result was obtained using the MTT assay method and incubate for 4 hours. The optical density absorbency which indicates cell viability, calculated by ELISA reader with a wavelength of 595nm. Cytotoxicity parameters using LC50 analyzed by probit analysis. The extract of Tithonia diversifolia leaf with the concentration of 0.5%, 0.25%, and 0.125% found to kill cell up to 50% and only the concentration 0.0625 of Tithonia diversifolia leaf extract with the highest cell viability. The LC50 value of Tithonia diversifolia leaf extract on bone marrow mesenchymal of rat is 0.167%.
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| Pubmed Style Plumeriastuti H, Kalista AV, Budiastuti , Effendi MH. Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. SRP. 2020; 11(9): 1008-1013. doi:10.31838/srp.2020.9.145 Web Style Plumeriastuti H, Kalista AV, Budiastuti , Effendi MH. Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. http://www.sysrevpharm.org/?mno=25718 [Access: March 29, 2021]. doi:10.31838/srp.2020.9.145 AMA (American Medical Association) Style Plumeriastuti H, Kalista AV, Budiastuti , Effendi MH. Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. SRP. 2020; 11(9): 1008-1013. doi:10.31838/srp.2020.9.145 Vancouver/ICMJE Style Plumeriastuti H, Kalista AV, Budiastuti , Effendi MH. Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. SRP. (2020), [cited March 29, 2021]; 11(9): 1008-1013. doi:10.31838/srp.2020.9.145 Harvard Style Plumeriastuti, H., Kalista, . A. V., Budiastuti, . & Effendi, . M. H. (2020) Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. SRP, 11 (9), 1008-1013. doi:10.31838/srp.2020.9.145 Turabian Style Plumeriastuti, Hani, Agesti Veva Kalista, Budiastuti, and Mustofa Helmi Effendi. 2020. Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. Systematic Reviews in Pharmacy, 11 (9), 1008-1013. doi:10.31838/srp.2020.9.145 Chicago Style Plumeriastuti, Hani, Agesti Veva Kalista, Budiastuti, and Mustofa Helmi Effendi. "Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method." Systematic Reviews in Pharmacy 11 (2020), 1008-1013. doi:10.31838/srp.2020.9.145 MLA (The Modern Language Association) Style Plumeriastuti, Hani, Agesti Veva Kalista, Budiastuti, and Mustofa Helmi Effendi. "Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method." Systematic Reviews in Pharmacy 11.9 (2020), 1008-1013. Print. doi:10.31838/srp.2020.9.145 APA (American Psychological Association) Style Plumeriastuti, H., Kalista, . A. V., Budiastuti, . & Effendi, . M. H. (2020) Cytotoxicity Test of Tithonia diversifolia Leaf Extract on Bone Marrow Mesenchymal Stem Cell (BMSC) of Rats Using MTT Assay Method. Systematic Reviews in Pharmacy, 11 (9), 1008-1013. doi:10.31838/srp.2020.9.145 |
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