Detection of some DNA repair systems of Klebsiella pneumonia isolated from patients with urinary tract infection

Abstract

Asmaa Khalid Hameed, Qasim Khlaif Abdullah, Aseel Khalid Hameed

Background: This study included the detection of the DNA repair systems of Klebsiella bacteria isolated from urinary tract infections. Ultraviolet rays and caffeine were used in this study, which has the ability to cause dimers in the DNA strip to detect the photo-DNA repair system and the eradication system.
Methodology : Klebsiella pneumonia exhibited 254 nm for Ultraviolet Radiation For a length of 100, 120 , 160 , 180 seconds, then take two ml of every A therapy of zero leaves 1 mL of them in the darkish and 1 mL of mild For an hour then I made a collection of comfort for her and planted it on Feeding nests and protecting the dishes with aluminum foil and incubated 37 for 24 hours.
Result : shows the effect of ultraviolet rays on the growth of Klebsiella bacteria in different time periods 100, 120,160,180 seconds, as it was noticed that the bacteria that were exposed to periods of radiation and were left in the dark decreased their growth compared to the bacteria that were left in the light, as we did not notice that they were affected much, this is for the period of irradiation of 100 seconds. Also, the growth decreases as the periods of irradiation increase, and this is due to the direct effect of killing the bacteria. We conclude from this that this bacteria has a photo-DNA repair system.