Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals
Abstract
Rana Hussein Raheema and Ban Hamid Qaddoori
Staphylococcus aureus, especially MRSA, has spread to all parts of the world and is becoming a significant concern in public health as one of the most common causes of nosocomial infections. This study aimed to assess the prevalence of MRSA toxin genes. The study was conducted in the medical microbiology laboratory of medicine college, Wasit University, Iraq, from January 2017 - June 2019. Fifty-seven isolates were isolated from blood culture, wound infections, urine culture, and nasal swabs, which were identified using microbiological and biochemical tests; antibiotic susceptibility was estimated by the clinical laboratory standards institute. Then the isolates were tested for Molecular screening to detect toxin genes. S. aureus was found in 33 (57.89%) isolates. MRSA was detected in 29 (87.8%) S. aureus isolated by cefoxitin, the results showed high susceptibility to imipenem (94%), ciprofloxacin (91%), clindamycin (91%) and nitrofurantoin (91%). In comparison, high resistance was observed with penicillin (94%). Molecular screening for eta, etb and tsst-1 genes, revealed positive amplification with 41.3%, 27.5 %, and 31 %, respectively. Fem A and luk-PV genes were detected in 44.8% and 10.3% of the isolates, respectively. There is a high prevalence of MRSA among different clinical isolates, which harbors toxin genes.
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| Pubmed Style Rana Hussein Raheema and Ban Hamid Qaddoori. Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. SRP. 2020; 11(10): 584-589. doi:10.31838/srp.2020.10.87 Web Style Rana Hussein Raheema and Ban Hamid Qaddoori. Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. http://www.sysrevpharm.org/?mno=2796 [Access: March 29, 2021]. doi:10.31838/srp.2020.10.87 AMA (American Medical Association) Style Rana Hussein Raheema and Ban Hamid Qaddoori. Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. SRP. 2020; 11(10): 584-589. doi:10.31838/srp.2020.10.87 Vancouver/ICMJE Style Rana Hussein Raheema and Ban Hamid Qaddoori. Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. SRP. (2020), [cited March 29, 2021]; 11(10): 584-589. doi:10.31838/srp.2020.10.87 Harvard Style Rana Hussein Raheema and Ban Hamid Qaddoori (2020) Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. SRP, 11 (10), 584-589. doi:10.31838/srp.2020.10.87 Turabian Style Rana Hussein Raheema and Ban Hamid Qaddoori. 2020. Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. Systematic Reviews in Pharmacy, 11 (10), 584-589. doi:10.31838/srp.2020.10.87 Chicago Style Rana Hussein Raheema and Ban Hamid Qaddoori. "Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals." Systematic Reviews in Pharmacy 11 (2020), 584-589. doi:10.31838/srp.2020.10.87 MLA (The Modern Language Association) Style Rana Hussein Raheema and Ban Hamid Qaddoori. "Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals." Systematic Reviews in Pharmacy 11.10 (2020), 584-589. Print. doi:10.31838/srp.2020.10.87 APA (American Psychological Association) Style Rana Hussein Raheema and Ban Hamid Qaddoori (2020) Detection of the Toxin Associated Genes of Methicillin-Resistant Staphylococcus Aureus Using a Multiplex PCR Assay in Wasit General Hospitals. Systematic Reviews in Pharmacy, 11 (10), 584-589. doi:10.31838/srp.2020.10.87 |
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