Detection the Nitric Oxide Levels upon Exposure to Interferon Gamma and Dexamethasone in Chicken Lymphoid Cancer Cells

Abstract

Israa Najm Abdullah Al-Ibadi, Abbas Hadi Jasim Al-Mahmoudi, Hiba Turkey Atyia

Nitric oxide (NO) was linked to disease inflammation severity; however, it has been shown to play roles in tumor growth and tumor suppression. The aim of this study was to investigate NO regulation upon exposure of chicken lymphoid cancer (DT40) cells to interferon gamma (INFG) and dexamethasone (DEX) at different concentrations. Pretreated-DT40 cells with 1µg INFG/ml of the nutrition media were managed by adding DEX concentrations at 0.1, 1, 10, 50µM, INF+DEX group. Moreover, one group was left with no INFG and DEX treatments, (NT) group, and other was received INFG only, (INFGO) group. After 24hrs of the treatment, the levels of NO were measured in the cells by using Griess System, and the cell proliferation rate was also recorded. The findings recorded significantly (pË‚0.05) the lowest levels of NO in the INFGO cells in a comparison with the NT and INF+DEX cells. Moreover, the results displayed significant (pË‚0.05) lower levels of NO in the INF+DEX cells when only compared to those identified from the NT cells. However, INF+DEX cells at the DEX concentration 10µM showed no change in the NO levels when only compared with those from the NT cells. For the proliferation of the cells, the outcomes unveiled significant (pË‚0.05) lower proliferation rates in the INF+DEX cells when compared to those identified from the NT and the INFGO cells. On the other hands, the proliferation rate of the INFGO cells was significantly (pË‚0.05) higher than that recorded in the NT and INF+DEX cells. Thus, interferon gamma and dexamethasone play important roles in regulating the levels of nitric oxide in the cells, and that high levels of NO may suppress the progression of the tumor cells.