Determination of Nitrite in Meat by Azo Dye Formation

Abstract

Tamathir Abbas Hamoudi, Aveen Faidhalla Jalal, Nabil Adel Fakhre.

A nitrite has been determined by developing a simple and sensitive spectrophotometric method which is involve the reaction between nitrite and Ï�-bromoaniline to form the corresponding diazonium ion which is afterward coupled with salbutamol in the presence of ammonia solution to form a yellow water-soluble and stable azo dye showing maximum absorption at 442 nm. A 0.1 - 3 μg NO2-/10 mL is follow of Beer's law, i.e., 0.1 - 3.0 ppm with Sandell's sensitivity index of 4.24 × 10-4 µg.cm-2 and ε, the molar absorptivity of 1.08606 × 105 L.mol-1. cm-1. The limit of detection and the limit of quantitation is calculated for determination of nitrite which is 0.0199 µg.ml-1 and 0.0274 µg.ml-1 severally. The applicability of this method has been tested by as saying nitrite in curing meat samples