Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets)
Abstract
Seemaa Hameed Ahmed
Purpose: To development a new high-pressure liquid chromatography (HPLC) technique for determination analysis of diphenhydramine hydrochloride (DPH) and ibuprofen (IBU) in tablet.
Methods: Chromatographic conditions were an isocratic system with C18- column (250 mm x 4.6mm,5µm), flow rate 1.0 ml/min and detector UV at 254 nm, And a mixture of 0.05 M of KH2PO4 buffer and acetonitrile (50:50) and this mixture, acetonitrile -triethylamine (70:30:0.3), v/v, pH 3,0) as mobile phase. The method was validated for selectivity, linearity, detection limit, LOQ, precision, and accuracy.
Results: Retention time of DPH and IBU were 4.3, 15.6 min, respectively. The method showed good selectivity, calibration curves were linear over the concentration range of 10– 100 μg/mL for DH, with correlation coefficient of 0.9997, and 50– 500μg/mL for IB, with correlation coefficient of 0.9996 accuracy was 99.46 - 100.20 and 100.01- 101.05 % DPH and IBU, respectively; precision (RSD) was < 1.0.
Conclusion: This method is sensitive, precise, highly selective, and accurate, and would be suitable for the simultaneous analysis of DPH and IBU in tablet dosage form. Since methanol is cheaper than acetonitrile, the application of the method may reduce the cost of analysis.
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| Pubmed Style Seemaa Hameed Ahmed. Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). SRP. 2020; 11(10): 225-229. doi:10.31838/srp.2020.10.38 Web Style Seemaa Hameed Ahmed. Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). http://www.sysrevpharm.org/?mno=16659 [Access: March 28, 2021]. doi:10.31838/srp.2020.10.38 AMA (American Medical Association) Style Seemaa Hameed Ahmed. Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). SRP. 2020; 11(10): 225-229. doi:10.31838/srp.2020.10.38 Vancouver/ICMJE Style Seemaa Hameed Ahmed. Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). SRP. (2020), [cited March 28, 2021]; 11(10): 225-229. doi:10.31838/srp.2020.10.38 Harvard Style Seemaa Hameed Ahmed (2020) Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). SRP, 11 (10), 225-229. doi:10.31838/srp.2020.10.38 Turabian Style Seemaa Hameed Ahmed. 2020. Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). Systematic Reviews in Pharmacy, 11 (10), 225-229. doi:10.31838/srp.2020.10.38 Chicago Style Seemaa Hameed Ahmed. "Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets)." Systematic Reviews in Pharmacy 11 (2020), 225-229. doi:10.31838/srp.2020.10.38 MLA (The Modern Language Association) Style Seemaa Hameed Ahmed. "Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets)." Systematic Reviews in Pharmacy 11.10 (2020), 225-229. Print. doi:10.31838/srp.2020.10.38 APA (American Psychological Association) Style Seemaa Hameed Ahmed (2020) Development of a Method for Determination of Diphenhydramine HCl and Ibuprofen in Pharmaceutical Preparations (Tablets). Systematic Reviews in Pharmacy, 11 (10), 225-229. doi:10.31838/srp.2020.10.38 |
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