Development of ZIC-HILIC Methods Using Ultraviolet Detection for determining 2-deoxyuridine in Human Serum

Abstract

Ashraf Saad Rasheed, Farah A. Rashid.

The work has been carried out the hydrophilic chromatographic sepa-ration of 2-deoxyuridine. Two handmade ZIC-HILIC columns with eluent (A-10%, acetate buffer 5 mM (pH 4.75); B-90%, of ACN) were used. The separation took place at 9 min with a flow rate of 0.75 mL/min. In application to the serum from humans, the quantification of 2-deoxyuridine was performed. The retention mechanism of the col-umns was studied in view of the use of 2-deoxyuridine for hydrophilic and anionic interactions. Such techniques are a valuable alternative to conventional 2-deoxyuridine separation methods. The LOD and LOQ were respectively 0.03 and 0.01 μg.mL-1 for two methods. The valida-tion parameters produced recovery rates from 96.95±0.83 to 97.39±0.63 with the precision of 1.44 ± 0.16 and 1.26 ± 0.25%, re-spectively, as a relative standard deviation.