DIFFERENCE BETWEEN EPITHELIAL AND COLLAGEN IN SECOND DEGREE BURNS BETWEEN HUMAN CORD MESENCHYMAL STEM CELL EXTRACT AND SILVER SULFADIAZINE IN THE MALE WHITE RAT (RATTUS NORVEGICUS)

Abstract

Evi Kurniawaty, Efrida Warganegara, Ni Made Ariyuliami Savitri.

Purpose: Silver sulvadiazine is the gold standard in topical therapy for burn healing. Currently, other therapies have been developed to help the wound healing process, including using human umbilical cord mesenchymal stem cell extract because stem cells can accelerate the formation of epithelium and collagen, thereby accelerating the wound healing process.
Research Methodology: This study used 27 male white rats Sprague Dawley divided into 9 treatment groups, groups K4, K14, and K28 were control groups, groups SC4, SC14, and SC28 were groups that were given stem cell extract therapy, and groups SSD4, SSD14, and SSD28 were groups that were given silver sulvadiazine therapy. On days 4, 14, and 28 the rats were euthanized to take their skins and made preparations by staining hematoxylin-eosin and observed the epithelial and collagen formation at 40x magnification.
Results: Mean epithelialization score on day 28 K28: 5.33, SC: 7.67, SSD28: 8. Mean collagen score on day 14 K14: 6.67, SC14: 8.67, SSD14: 8. Mean collagen score on day 28 K28: 5, SC28: 4, SSD28: 3.67. There was a significant difference in epithelial thickness on day 28 and the amount of collagen on days 14 and 28