Dynamic of Intracellular Cytokine's Production in Patients with Recurrent Implantation Failure and Thin Endometrium in In Vitro Fertilization Program

Abstract

Almagul Medeubayevna Kurmanova, Gaini Anartayeva*, Saltanat Baikoshkarova, Sonny Stetson, Gaukhar Medeubayevna Kurmanova, Nagima Mamedalieva, Kudaibergenov

The article presents results of research of 25 patients with recurrent implantation failure after In Vitro Fertilisation (IVF) and the "thin endometrium" in comparison with 30 patients without reproductive loss and normal endometrial thickness. Dynamics of intracellular production of γ-INF, IL-1 and IL-10 by cytotoxic endometrial lymphocytes was studied by flow cytometry. It was found that before treatment in patients with “thin endometrial” syndrome and recurrent implantation failure after IVF there were a significant (18-fold) decrease in the level of CD8+ cytotoxic/suppressor lymphocytes (p<0.01), a 3-fold decrease in the level of CD56+ cells, as well as a sharp inhibition of the level of intracellular production of cytokines- γ-INF (10 times), IL-1 (10 times) and IL-10 (3 times); there was a sharp decrease in intracellular production of γ-interferon by CD8+ lymphocytes-almost 35 times, by CD16+ lymphocytes-6,8 times and by CD56 + lymphocytes-16 times. During complex therapy including the elimination of pathogens, personalized hormonal therapy with progesterone (Gynoprogest), there were found that the thickness of the M-echo endometrium increased from 6,82 ± 1.3 mm to 13.0 ± 1.5 mm; and there was also a positive dynamics of intracellular cytokine production with a significant (p<0.01) increase in the level of IL-1 and IL-10, as well as the level of γ-IFN by endometrial lymphocytes.