Effect of Beluntas Leaf Extract (Pluchea indica) on Oral Mucosal Wound Healing in Terms of Density of Inflammatory Cells and Collagen

Abstract

Natallia Pranata, Gabriella Evita Datu Boli, Rabecka Sinta, Vinna Kurniawati Sugiaman.

Traumatic injury is one of the acute injuries that often occur in the oral cavity. The wound healing process consists of the phases of hemostasis, inflammation, proliferation, and tissue remodeling. Some of the active components of herbal plants can help speed up the wound healing process and have anti-inflammatory effects. Beluntas leaves which contain flavonoids, tannins, and saponins have the potential to be used as herbal medicine because they have anti-inflammatory effects, but standardization is needed first. The purpose of this study was to determine the effect of beluntas leaf extract (Pluchea indica) on wound healing in the oral mucosa in terms of the number of inflammatory cells and collagen in tissue preparations stained with Hematoxylin Eosin. The method in this research is a descriptive laboratory experimental research design with the post-test only control group design. The number of samples of 30 Wistar rats, divided into 6 groups that had been treated and terminated on the 3rd and 6th days were then continued to the microscopic stage by making paraffin blocks, making preparations, and staining using Hematoxylin Eosin (HE). The assessment of HE streaks was semiquantitative, all data from the research results were statistically tested using the Kruskal-Wallis Test. Based on the test results, it is known that there are significant differences in the effect of beluntas leaf extract on the density of inflammation and collagen cells compared to the negative control and the positive control (povidone iodine). The conclusion from this study is that it can be seen that beluntas leaf extract can help accelerate the process of wound healing in the oral mucosa as indicated by a significant decrease in the density of inflammatory cells and an increase in collagen density.