Effect of Curcumin on sFlt-1 and PlGF Concentration in Preeclampsia Induced HUVEC Cell Line

Abstract

Cut Meurah Yeni, Prima Nanda Fauziah, Ani Melani Maskoen, Rovina Ruslami, Johannes Mose

Preeclampsia is a multisystem disorder characterized by hypertension and proteinuria. Elevated soluble fms-like tyrosine kinase 1 (sFlt-1) and reduced placental growth factor (PlGF) levels are documented in preeclampsia patients. Oxidative stress is known to play a role in the etiopathogenesis of preeclampsia. Hence, antioxidants as physiological protective agents can be used in the treating preeclampsia. In this study, we assessed the antioxidant properties of curcumin in HUVEC cell line cultured in serums collected from normal and preeclamptic pregnant women. The levels of sFlt-1 and PlGF were measured with ELISA. We found that sFlt-1 level and sFlt-1/PlGF ratio decreased significantly (p<0.05) while PIGF level increased significantly (p<0.05) after the curcumin administration with concentrations ranged from 0.977–250 µg/mL. The levels of sFlt-1, PlGF, and sFlt-1/PlGF ratio in preeclampsia-induced cell were equal to those in normal cell after the addition of 7.813 µg/mL curcumin after 24 h incubation. Similar results were obtained for samples with 48 h incubation, in exception with PlGF level in preeclampsiainduced cell that required 31.250 µg/mL curcumin to be equal to that in normal cell line. In conclusion, curcumin to be used in treating preeclampsia, by restoring the balance between proangiogenic factor (PlGF) and antiangiogenic factor (sFlt-1). However, further studies are warranted to elucidate this potency.