Effectiveness of Autoclave Combination Treatment with Andosol Soil to Decrease the Number of Bacillus Cereus
Marsum, Anies, Bagoes Widjanarko, Nur Endah Wahyuningsih.
Strict regulations and permits in managing medical waste create dependency on third party. This has an impact on medical waste especially syringe medical waste which must be piled up waiting for the collection process. Improper handling medical waste may cause accidents and disease transmission. Soil has been known to be used as an antibacterial in medicine, but there is still little in the process of application in medical waste management. This study aims to determine the effectiveness of the use of autoclaves combined with andisol soils to decrease the number of bacterial colonies of Bacillus cereus. This study uses a true experiment design using a completely randomized design. The research was carried out starting from the isolation of bacteria from syringe medical waste and rejuvenation on sterile media to test the effect of the application treatment on a laboratory. Anova Repeated Measure Test (Ranova) at alpha 0.05 was used to determine differences in the number of bacteria that grew in the media before and after treatment. The results of this study indicate the combination treatment of autoclaves in the variation of contact time with andosol soils at variations in concentration produced the difference (p <0.001) with the number of bacterial colonies Bacillus cereus between 290,000-340,000 CFU / ml and the effectiveness of all treatments reaches 100%. At the end of this study, a combination of autoclave treatment at a temperature of 121 0C was taken for 15 minutes with andosol soil treatment at a concentration of 45% with a contact duration of 2 minutes that could be applied independently at the hospital.