Estimation of Pollination in Mahogany Revealed by Microsatellite Markers : Case in South Sulawesi, Indonesia

Abstract

Siti Halimah Larekeng, Gusmiaty, Yuni Fitri Cahyaningsih, Mirza Arsiaty Arsyad, Wanti Mustika Sari, Muhammad Restu, Astuti Arif

Research Highlights: Pollen dispersal can be used to design and manage seed orchards of Mahogany to fit the breeding demand. Pollen dispersal and mating system in mahogany can be elucidated through pollen dispersal pattern analysis based on genetic structure. Background and Objective: Microsatellite is a co-dominant genetic marker that has been widely applied for DNA analysis. The objective is this work were to to determine specific primers for parentage analysis, to calculate the distance of pollens travel from donor pollen to female recipient, to estimate the frequency of pollination, and to evaluate the proportion of selfing and outcrossing in mahogany. Materials and Methods: The field activity was done by collecting leaf samples at genetic resource area of Sulawesi BPTH, South Sulawesi, Indonesia. Genetic molecular analysis were conducted at Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Universitas Hasanuddin, Indonesia. Results: The study indicated that the suitable primers for parentage analysis were sm05, sm18, and sm48. Pollens could travel up to 83 m apart from the evaluated female parents, and the highest frequency of pollination was 22 at 20 to 30 m. The proportion of outcrossing was 95% which indicated that mahogany trees in this evaluated area tend to outcross pollinate. Conclusion: Proportion of crossing was 95% which indicated crossing pollination dominating the mahogany population in genetic resource area Gowa. SSR primers screening generating polymorphic bands were sm03, sm18, and sm48. Highly variable microsatellite markers have facilitated a direct genetic approach to measuring gene flow based on parentage analysis.