Estimation of Truffles extracts and study the biological efficacy of Ethyl 6-methyl-2-oxo-4-(2-thienyl)-1,2,3,4-tetrahydropyrimidine-5-carboxylate (EMOTTC) as one of the extracts against ophthalmic bacteria

Abstract

Rana D. S. ALkamil, Dawood CH. Al-Bahadily, Rasool Chaloob, Falah Hassan Shari, H. N. K. AL-Salman

CONTEXT: The study indicated to the biological effectiveness of the compound is Ethyl 6-methyl-2-oxo-4- (2-thienyl) -1,2,3,4-tetrahydropyrimidine-5-carboxylate (EMOTTC), as antibacterial which is one of the six compounds extracted from truffles. A number of organic solvents have been used as CH3OH, C2H5OH, CHCl3, C4H8O2, and n-C6H14. The highest percentage of the extract was obtained in the extraction process when using methanol alcohol and the lowest percentage when using chloroform. OBJECTIVE: The biological efficacy of (EMOTTC) was investigated against different types of bacteria by taking higher Concentration of this compound and the lowest concentration of the compound. MATERIALS AND METHODS: The purpose of the manuscript was to study bacterial inhibitory activity using MacConkey Agar as an agricultural medium. (EMOTTC) showed excellent bioavailability against different types of Gram-positive and positive bacteria. The EMOTTC compound has a strong effect on pathogenic bacteria, especially Staphylococcus aureus, Haemophilus influenzae, and Pseudomonas aeruginosa, which indicates that this compound can have multiple drugs uses as an alternative to antibiotics and sterilizers. RESULTS: Using the GC-Mas Spectrum technique, all the bio-organic compounds were diagnosis in the organic layer of the extract, then several methods were performed to determine the type and concentration of each organic compound alone. Spectroscopy of (EMOTTC) was studied, and its biological activity against Gram-positive and Positive bacteria was studied. CONCLUSIONS: Six compounds were detected in Truffle extraction by GC-Mas technology. The same technique demonstrated the ability to isolate (EMOTTC) using different solvents and the effect of this biologically active compound was studied against three Types of bacteria that cause eye infections and ophthalmia.