Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells
Abstract
Happy Kurnia P, Rizâq Threevisca C, Dhanang Puruhita TR, Muhammad Nazhif Haykal.
Cervical cancer belongs to the main cancer group experienced by women in developing countries, including Indonesia. An estimated 95% of cervical cancer is related to human papillomavirus (HPV) infections. HPV has oncoprotein E6 and E7, which can degrade p53, a tumor-suppressor gene, subsequently interfering with the activity of the p21 and caspase-3 genes and disrupting apoptosis and cell proliferation activity. Eugenol (4-allyl-2-methoxyphenol) is an active substance derived from cloves (Syzygium aromaticum) that has been studied for its anti-cancer capability. Here, eugenol was optimized with modifications for nanoparticle encapsulation with chitosan (nano-eugenol) to improve its bioavailability and stability, reduce its toxicity, and also to improve its anti-cancer capabilities. This research examined the effect of nano-eugenol on HeLa cell viability using trypan blue method, HeLa cell cycle analyzed using flow cytometry. Eugenol was successfully loaded onto nanoparticles sized 250 nm and 351 nm and with a polydispersity index of <0.5. Compared to eugenol alone, the nano-eugenol reduced cell viability (p < 0.0001) and inhibited the cell cycle in G0/G1 (p < 0.0001) effectively. We may conclude that modifying eugenol with chitosan encapsulation nanoparticles can optimize the anti-cancer effect of eugenol itself on cancer cells.
| How to Cite this Article |
| Pubmed Style P HK, C RT, TR DP, Haykal MN. Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. SRP. 2021; 12(1): 692-699. doi:10.31838/srp.2021.1.100 Web Style P HK, C RT, TR DP, Haykal MN. Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. http://www.sysrevpharm.org/?mno=42111 [Access: March 30, 2021]. doi:10.31838/srp.2021.1.100 AMA (American Medical Association) Style P HK, C RT, TR DP, Haykal MN. Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. SRP. 2021; 12(1): 692-699. doi:10.31838/srp.2021.1.100 Vancouver/ICMJE Style P HK, C RT, TR DP, Haykal MN. Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. SRP. (2021), [cited March 30, 2021]; 12(1): 692-699. doi:10.31838/srp.2021.1.100 Harvard Style P, H. K., C, . R. T., TR, . D. P. & Haykal, . M. N. (2021) Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. SRP, 12 (1), 692-699. doi:10.31838/srp.2021.1.100 Turabian Style P, Happy Kurnia, Riz'q Threevisca C, Dhanang Puruhita TR, and Muhammad Nazhif Haykal. 2021. Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. Systematic Reviews in Pharmacy, 12 (1), 692-699. doi:10.31838/srp.2021.1.100 Chicago Style P, Happy Kurnia, Riz'q Threevisca C, Dhanang Puruhita TR, and Muhammad Nazhif Haykal. "Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells." Systematic Reviews in Pharmacy 12 (2021), 692-699. doi:10.31838/srp.2021.1.100 MLA (The Modern Language Association) Style P, Happy Kurnia, Riz'q Threevisca C, Dhanang Puruhita TR, and Muhammad Nazhif Haykal. "Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells." Systematic Reviews in Pharmacy 12.1 (2021), 692-699. Print. doi:10.31838/srp.2021.1.100 APA (American Psychological Association) Style P, H. K., C, . R. T., TR, . D. P. & Haykal, . M. N. (2021) Eugenol Nanoparticle Encapsulated Chitosan Enhances Cell Cycle Arrest in HeLa Human Cervical Cancer Cells. Systematic Reviews in Pharmacy, 12 (1), 692-699. doi:10.31838/srp.2021.1.100 |
Abstract
PDF
How to cite this article
Related Records