Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry
Abstract
Seher A. Almadany, Khalida K. Abbas Al-Kelaby, Sarmad N. Gany, Hussein A. Abdul Hussein
The flow cytometry capability for fast and individualized analysis of a big number of cells would seem to make it typically selected for the cell death assessment. It offers the capability to revise large numbers cells separately rather than a mixed population, and investigates concurrently many proteins expression like cell specific markers and apoptotic indicators. Annexin V assay is one of the most widely used approach to detect apoptosis as it is easy-to-use, fast , needs no fixation and allows a large number of cells to be analyzed at any one time. Annexin assay is ideal for solitary cell suspensions and so flow cytometry. In this study we try to evaluate the apoptotic effect of the investigational drug TAN on human cancer cell line HCT116 in comparison with the triazoles: itraconazole (ITC), difenoconazole (DIF), epoxyconazole (EPO), and the standard anticancer agent’s doxorubicin (DOX) and 5-fluorouracil (5FU) by flow cytometry. To assess the apoptotic effect of the triazole analog TAN on human colorectal cancer cell line HCT116using annexin V dye via flow cytometry assay. The results of flow cytometry reveal a significant differences between treated and untreated HCT116 cells in all drugs based on mean ±Std. in all drugs that are used in this study , TAN showed a significant apoptotic effect after DOX ,DIF and EPO with P value = . 0005, the following conclusions as the TAN has significant and promising anticancerrole through induction of apoptosis in HCT116 cells and flow cytometry is a reliable technique to measure the apoptotic percentages in cells.
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| Pubmed Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP. 2019; 10(1): 214-219. doi:10.5530/srp.2019.1.37 Web Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. http://www.sysrevpharm.org/?mno=302644975 [Access: March 28, 2021]. doi:10.5530/srp.2019.1.37 AMA (American Medical Association) Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP. 2019; 10(1): 214-219. doi:10.5530/srp.2019.1.37 Vancouver/ICMJE Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP. (2019), [cited March 28, 2021]; 10(1): 214-219. doi:10.5530/srp.2019.1.37 Harvard Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein (2019) Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. SRP, 10 (1), 214-219. doi:10.5530/srp.2019.1.37 Turabian Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. 2019. Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. Systematic Reviews in Pharmacy, 10 (1), 214-219. doi:10.5530/srp.2019.1.37 Chicago Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. "Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry." Systematic Reviews in Pharmacy 10 (2019), 214-219. doi:10.5530/srp.2019.1.37 MLA (The Modern Language Association) Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein. "Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry." Systematic Reviews in Pharmacy 10.1 (2019), 214-219. Print. doi:10.5530/srp.2019.1.37 APA (American Psychological Association) Style Seher A. Almedeny , Khalida K. Abbas Al-Kelaby , Sarmad N. Gany , Hussein A. Abdul Hussein (2019) Evaluation of the apoptotic effect of the triazole analog TAN on human colorectal Cancer cell line HCT 116 by flow cytometry. Systematic Reviews in Pharmacy, 10 (1), 214-219. doi:10.5530/srp.2019.1.37 |
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