Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice
Abstract
Saikhu Akhmad Husen, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, Win Darmanto.
Introduction: Diabetes mellitus (DM) is a chronic disease with diverse and complex metabolic disorders. DM can be identified with hyperglycemic conditions. In 2017, Indonesia ranked sixth in the world for the highest number of diabetic patients after China, India, United States, Brazil, and Mexico.
Objective: This study aims to determine the combined effect of alginate extract from Sargassum duplicatum and Sargassum ilicifolium with okra (Abelmoschus esculentus) and mangosteen (Garcinia mangostana) pericarp extracts to ameliorate the wound width process, the number of neutrophil cells, macrophages, fibroblasts, fibrocytes, and collagen density in streptozotocin-induced diabetic mice.
Methods: This study used male mice (strain BALB/C, weighting 30±5 g each) which were grouped into four groups; normal control with water only; diabetic control group; combination treatment group with S. duplicatum extract, A. esculentus extract, and G. mangostana extract; and combination treatment group with S. ilicifolium extract, A. esculentus extract, and G. mangostana extract. The diabetic control group and combination treatment groups were injected with multiple low doses of streptozotocin (STZ) at 30 mg/kg BW intraperitoneally. The combination extract dose given in treatment groups was 50 mg/kg BW. Measurement of fasting blood sugar levels was done before and after the STZ injection. The mice’s skin was cut in the gluteal section as long as 1 cm, then treated topically in three different time periods namely 3, 7, and 14 days. Histological preparation and analysis were conducted on the 15th day.
Results: The administration of A. esculentus extract and G. mangostana extract combined with S. duplicatum and S. ilicifolium increased wound healing indicator rate, decreased neutrophile cell count (NCC) and fibrocyte cell count (FCCC), and increased collagen synthesis as an extracellular matrix (ECM). The treatments could restore the open wound healing process of diabetic mice to their normal state. There were no significant differences in macrophage cell count (MCC) and fibroblast cell count (FBCC). The histopathology showed that the combination treatment groups also could accelerate the wound healing process in mice skin.
Conclusion: In summary, this study addressed that the administration of A. esculentus extract and G. mangostana extract combined with S. duplicatum and S. silicifolium extracts could ameliorate open wound healing in diabetic mice.
| How to Cite this Article |
| Pubmed Style Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP. 2020; 11(9): 888-892. doi:10.31838/srp.2020.9.129 Web Style Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. http://www.sysrevpharm.org/?mno=22602 [Access: March 30, 2021]. doi:10.31838/srp.2020.9.129 AMA (American Medical Association) Style Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP. 2020; 11(9): 888-892. doi:10.31838/srp.2020.9.129 Vancouver/ICMJE Style Husen SA, Syadzha MF, Setyawan MF, Pudjiastuti P, Ansori ANM, Susilo RJK, Hayaza S, Winarni D, Darmanto W. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP. (2020), [cited March 30, 2021]; 11(9): 888-892. doi:10.31838/srp.2020.9.129 Harvard Style Husen, S. A., Syadzha, . M. F., Setyawan, . M. F., Pudjiastuti, . P., Ansori, . A. N. M., Susilo, . R. J. K., Hayaza, . S., Winarni, . D. & Darmanto, . W. (2020) Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. SRP, 11 (9), 888-892. doi:10.31838/srp.2020.9.129 Turabian Style Husen, Saikhu Akhmad, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, and Win Darmanto. 2020. Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. Systematic Reviews in Pharmacy, 11 (9), 888-892. doi:10.31838/srp.2020.9.129 Chicago Style Husen, Saikhu Akhmad, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, and Win Darmanto. "Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice." Systematic Reviews in Pharmacy 11 (2020), 888-892. doi:10.31838/srp.2020.9.129 MLA (The Modern Language Association) Style Husen, Saikhu Akhmad, Muhammad Farraz Syadzha, Muhamad Frendy Setyawan, Pratiwi Pudjiastuti, Arif Nur Muhammad Ansori, Raden Joko Kuncoroningrat Susilo, Suhailah Hayaza, Dwi Winarni, and Win Darmanto. "Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice." Systematic Reviews in Pharmacy 11.9 (2020), 888-892. Print. doi:10.31838/srp.2020.9.129 APA (American Psychological Association) Style Husen, S. A., Syadzha, . M. F., Setyawan, . M. F., Pudjiastuti, . P., Ansori, . A. N. M., Susilo, . R. J. K., Hayaza, . S., Winarni, . D. & Darmanto, . W. (2020) Evaluation of the Combination of Sargassum Duplicatum, Sargassum Ilicifolium, Abelmoschus Esculentus, and Garcinia Mangostana Extracts for Open Wound Healing in Diabetic Mice. Systematic Reviews in Pharmacy, 11 (9), 888-892. doi:10.31838/srp.2020.9.129 |
Abstract
PDF
How to cite this article
Related Records