Fibroblast Viability Test Toward Red Dragon Fruit (Hylocereus polyrhizus) Peel Ethanolic Extract
Annabella Natasha Kylanel, Vinna Kurniawati Sugiaman, Natallia Pranata.
The nutritional content of red dragon (Hylocereus polyrhizu) fruit is not only limited to the flesh, the peel contains polyphenols and antioxidant activity along with the antiproliferative effects that are higher than the flesh. Plant bioactive constituents is belief to have various benefits with minimum side effects but yet not standardized. Phytochemical and viability test are needed to analyze the toxic concentration and 50% inhibition concentration (IC50) from Hylocereus polyrhizus peel 70% ethanolic crude extract.
Materials and methods: Qualitative phytochemical test was determined using modified Farnsworth method. Fibroblast cells (3T3BALB/C) given seven different extract (500, 250, 125, 62.5, 31.25, 15.625, and 7.8125 Î¼g / mL) concentrations. Analysis using colorimetric MTS assay with triplicate. The value of formazan absorbance indicated the viability of the cells.
Results: Flavonoids, saponin, phenols, tannins, triterpenoids, terpenoids, and alkaloids were presence. Statistical data analysis obtained using ANOVA where each group has significantly different effect (p < 0.05) and IC50 value was obtained 1576.867 Î¼g / mL, higher than the concentration group that was planned before.
Conclusion: RDFP 70% ethanol extract proven to be not toxic, particularly at a concentration below 250 Î¼g / mL. Meanwhile, the IC50 higher than the 100% concentration.