Gene expression analysis of CAR2 Effects of n-butanol extract of celery STZ-Indused Diabetic female Rat

Abstract

Zahida Miran Hussein Alqayyim

An change of anti-hyperglycemiain streptozotocin-induced diabetic rats and gene expression (car2) as investigated for the active celery n-butanol extraction (Apium graveolens) crop. Diabetes mellitus induced on pregnant rats, their fetus and organogenesis of the pancreas at each time (14, 16, and 18) days post gestation. This research is performed in (75) healthy as adult virgin females Wistar albino rats (Rattus norvegicus), diabetes mellitus induced one intraperitoneal injection in (36) females before mating using streptozotocin (60 mg / kg animal body weight), instead divided in two classes one intraperitoneal injection. of them treated with n-butanolic celery seeds extract (60 mg/kg of body weight daily), while the other drink tap water, both of them included (18) females. Other (36) females use as control groups, which also divided in to two groups first one treated with the same dose of the extract and the second one drink tap water, both of them included (18) females With respect of molecular study on gene expression of fetus was referred to highly decrease in level of gene expression for genes (CAR2) of diabetic groups with a great increased of it in pregnant rats that treated with extract,whereas the greater value reported byCAR2 genes, alsoshowed clearly increase in diabetic rats that treated with extract, all this changes of the level of gene expression referred to increased through gestation age. Data on gene expression of (CAR2) in fetuses showed great increase in pregnant rats treated with extract, while the level of gene expression reported highly decrease in diabetic rats and clearly increase in diabetic pregnant rats treated with extract. All these changes increased with the time of gestation and reported higher value in 18th day postgestation.The study also included the identification of effective compounds of extract that used as a possible treatment for diabetes in pregnant mothers,included medical important compounds