Hepato Pancreatic Protective Potentials of Iraqi Aqueous Allium cepa Extract against Low Double Doses of Alloxan Induced Oxidoreductive Stress Mediated Diabetes mellitus in Rats
Ajwad Awad Muhammad Assumaidaee, Nathera M. Ali, Zaid O Ibraheem, Abbas Jasim Mohammed.
Biochemical and histopathological assessment of using aqueous Allium cepa extract against oxidoreductive stress and hepato pancreatic cytoarchitectural changes induced by two low double doses of alloxan (75mg/kg body weight/dose) was studied. Forty eight male rats were divided into 4 main groups (n=12), viz; Group 1 (CN – Gr) which was treated as the negative control and was allowed for the free access to the rodents chow and the tap water for the whole study period, Group 2 or the (AACE – Gr) which was assigned as the herb control received a daily oral dose of 1 ml of aqueous Allium cepa extract/100g body weight that was equivalent to (0.45 g/100 g body weight), Group 3 or (ALX-Gr), the alloxan-injected group which was given alloxan at (75 mg/kg body weight) on the first day of the first and 5th week of the experimental period and Group 4 (ALX+AACE-Gr) which was injected by double doses of alloxan as G3 and received a daily oral dose of 1 ml of aqueous Allium cepa extract/100 g body weight that was equivalent to (0.45 g/100 g body weight). Each of the mentioned groups was subdivided into two halves (n=6) that one was exsanguinated on day 28 (subgroup A) while the rest were kept till the end of the study period (56 days) (Subgroup B). Serum malondialdehyde concentration, total oxidative stress and oxidative stress index as oxidoreductive stress biomarker, activities of certain anti-oxidoreductive stress enzymes (glutathione peroxidase, super oxide dismutase and catalase) and concentration of reduced form glutathione with total antioxidative stress capacity and lipid profile were estimated. In Addition, liver and pancreas histopathological findings were evaluated.
In this study, the continuous daily administration of aqueous Allium cepa extract significantly protect against deleterious impact of free radicals action and thus preserving hepatic and pancreatic cellular restoration and architecture. Taken together, the biochemical and histopathological findings of the current study indicate a hepato-pancreatic protective effects and antidiabetic efficacy of aqueous Allium cepa extract against time and dose dependent manner of oxidoreductive stress mediated alloxan diabetogenicity in rats