Histological Changes and Some Biochemical Analysis in Male Rats Exposed High Fat Diet

Abstract

Yarub Modhar Al-Qazwinia, Muna Hussain AL-Aamelib, Kadhim S. Kadhimb

Fats enriched diets greatly raise the level cholesterol level in body tissues. The current study aims to studying the effects of dietary cholesterol on the histological changes in the testis of the male rats& physiological aspects of lipid profile in relation with cholesterol diet. Ten male of albino rats were subdivided into two groups (n=5): group 1 was given 1 ml of normal saline & considered as control group(G1), while the second group treated with enriched -cholesterol nutriment once at a dosage of 1.5 mg / k g body weight extent to 8 weeks & considered as treated group(G2) . The tissues of testes were obtained & stained with H & E stain. Blood samples were withdrawn after starvation of the animals at night after the end of the experiment to study the following parameters: measurement the intensification of ( T C ), ( T A G ), ( H D L ), ( L D L ) & ( V L D L ). Result showed the presence of a significant increase (p < 0. 0 5) in the concentrations of TC, TAG, LDL, VLDL & significant decrease (p < 0 .0 5) in the concentrations of HDL in processed group (G2) compared with the control group (G 1). Histological results from rats treated with cholesterol have showed histological abnormalities of testis structure represented as reduced sperm cells and thickened layers intertubular connective tissue, distorted Leydic cells, and thickened blood-vessel walls. is concluded from the current study that High-cholesterol diet causes defects in the in-testis tissue with alternation in the lipid profile in male albino rats.