HPTLC and GC-MS Analyses of Biologically Active Extracts and fractions from Premna latifolia Roxb Leaves reveal Anti-inflammatory and Analgesic Effects

Abstract

Rajesh Kumar41809*, Ashutosh Kumar41810, Ajay Kumar41811 and Manish Singh41812

Introduction: The goal of this study was to look at the various phyto-constituents in the ethyl acetate fraction of Premna latifolia methanolic leaves extract (MEPL) and see if they might be used for central/peripheral analgesia and inflammation.

Method: The goal of this study was to look at the various phyto-constituents in the ethyl acetate fraction of Premna latifolia methanolic leaves extract (MEPL) and see if they might be used for central/peripheral analgesia and inflammation. The analysis was carried out using GC-MS and HPTLC equipment. Premna latifolia methanolic leaves extract was tested for various activities at doses of 100 mg/kg, 200 mg/kg, and 300 mg/kg.

Results: Squalene (13.57%), Ergosta-5,7,9(11), 22-tetraen-3-ol, (3-beta, 22 E)-(0.15%), Stigmasterol(3.73%), gamma-Sitosterol (10.13%), Lupeol (0.33), beta-Amyrin (2.27%), alpha-Amyrin (2.05%), gamma (0.72%), GC- MS analysis revealed that they were prominent secondary metabolites. Premna latifolia dosages reduced central analgesia, as well as carrageenan and histamine- induced inflammation, in a dose-dependent manner (p<0.05).

Conclusion: In numerous animal models, key phytoconstituents of MEPL have been found to have significant analgesic and anti-inflammatory effects.