Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection
Abstract
Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim.
This study aims to introduce a novel method that included zwitterion chromatography-hydrophilic interaction chromatography with a UV detection system for analysis and identification of the fisetin in black and green teas. Flavonoids like fisetin have been shown to have multiple beneficial effects on human health that are available in many fruits, beverages, herbs, vegetables, and nutritional supplements herbs are the main source of flavonoids. Hydrophobic and hydrophilic interactions forming a fisetin mechanism to distinguish mixed mode. The validated process has been successfully used for the extraction test in herbs. Fisetin has been separated in the ZIC-HILIC column using acetonitrile and sodium acetate buffer as the mobile phase. The results showed that the HILIC mode was simple and effective, and could be used to identify the fisetin content in samples of black tea leaves and green tea leaves. The calibration curve was produced in commercial ZIC-HILIC column and linear range (0.01-7 μgmL-1), RSD% (1.32), LOD (0.008 μgmL-1), LOQ (0.024 μgmL-1).
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| Pubmed Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. SRP. 2021; 12(2): 289-294. doi:10.31838/srp.2021.2.39 Web Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. http://www.sysrevpharm.org/?mno=30958 [Access: March 29, 2021]. doi:10.31838/srp.2021.2.39 AMA (American Medical Association) Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. SRP. 2021; 12(2): 289-294. doi:10.31838/srp.2021.2.39 Vancouver/ICMJE Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. SRP. (2021), [cited March 29, 2021]; 12(2): 289-294. doi:10.31838/srp.2021.2.39 Harvard Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim (2021) Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. SRP, 12 (2), 289-294. doi:10.31838/srp.2021.2.39 Turabian Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. 2021. Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. Systematic Reviews in Pharmacy, 12 (2), 289-294. doi:10.31838/srp.2021.2.39 Chicago Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. "Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection." Systematic Reviews in Pharmacy 12 (2021), 289-294. doi:10.31838/srp.2021.2.39 MLA (The Modern Language Association) Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim. "Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection." Systematic Reviews in Pharmacy 12.2 (2021), 289-294. Print. doi:10.31838/srp.2021.2.39 APA (American Psychological Association) Style Salsal Kamal Abdulrahman, Ashraf Saad Rasheed, and Ameen Waleed Qassim (2021) Identification And Determination Of Fisetin In Black And Green Teas By ZIC-HILIC Column Coupled With UV Detection. Systematic Reviews in Pharmacy, 12 (2), 289-294. doi:10.31838/srp.2021.2.39 |
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