In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis
Abstract
Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide
Hibiscus sabdariffa L. or roselle calyx has been known to have biological activities including antioxidant and antibacterial. In this research, we examine the antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) as scavenging agent and the anti-mycobacterial activity towards clinical isolate of multidrug-resistant Mycobacterium tuberculosis (MDR-TB). Roselle calyces were dried and extracted through infusion methods using distilled water, and maceration methods using 50% ethanol, within the ratio of sample (1): solvent (10), followed by spray-drying using maltodextrin as diluting agent. The total phenolic content (TPC) was analyzed using Folin-Ciocalteu method calculated as gallic acid equivalent (GAE), antioxidant activity was analyzed using DPPH method, and anti-mycobacterial activity was assay using Microscopic Observation Drug Susceptibility (MODS) method. As the results, TPC of infusion extract and ethanolic extract were 17.70+0.7 mg GAE/g and 13.15+0.3 mg GAE/g, respectively and IC50 toward DPPH were 0.88 mg/ml for infusion extract and 3.78 mg/ml for ethanolic extract, respectively. Anti-mycobacterial activity of both extracts expressed as Minimum Inhibitory Concentration (MIC) were 10 mg/ml toward clinical isolate of MDR-TB.
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| Pubmed Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. SRP. 2020; 11(5): 36-39. doi:10.31838/srp.2020.5.07 Web Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. http://www.sysrevpharm.org/?mno=106525 [Access: March 29, 2021]. doi:10.31838/srp.2020.5.07 AMA (American Medical Association) Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. SRP. 2020; 11(5): 36-39. doi:10.31838/srp.2020.5.07 Vancouver/ICMJE Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. SRP. (2020), [cited March 29, 2021]; 11(5): 36-39. doi:10.31838/srp.2020.5.07 Harvard Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide (2020) In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. SRP, 11 (5), 36-39. doi:10.31838/srp.2020.5.07 Turabian Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. 2020. In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. Systematic Reviews in Pharmacy, 11 (5), 36-39. doi:10.31838/srp.2020.5.07 Chicago Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. "In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis." Systematic Reviews in Pharmacy 11 (2020), 36-39. doi:10.31838/srp.2020.5.07 MLA (The Modern Language Association) Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide. "In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis." Systematic Reviews in Pharmacy 11.5 (2020), 36-39. Print. doi:10.31838/srp.2020.5.07 APA (American Psychological Association) Style Sartini Sartini, M Natsir Djide, Gemini Alam, Andi Arjuna, Nana Juniarti N Djide (2020) In Vitro Antioxidant and Anti-Mycobacterial Activities of Roselle (Hibiscus sabdariffa L.) Calyx Extract against Clinical Isolate of Multidrug Resistant Mycobacterium tuberculosis. Systematic Reviews in Pharmacy, 11 (5), 36-39. doi:10.31838/srp.2020.5.07 |
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