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In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation

Abstract

Dzul Fadly, Clara M. Kusharto, Lilik Kustiyah, Pipih Suptijah, Yuges Saputri Muttalib, Bohari.

The study was aimed to evaluate the lipid peroxides blockage capability of carboxymethyl chitosan (CMCTS) from silkworm pupa. It was an experimental study. CMCTS was produced from silkworm pupa. CMCTS of silkworm pupa was subjected to be analysed the toxicity through Brine Shrimp Lethality (BSL) assay, antioxidant activity through DPPH assay, and lipid peroxides inhibitory capacity in human plasma through TBARS assay. The result of our screening indicated that 24 h of treatment of CMCTS had a low impact on the percentage of Artemia salina L. nauplii lethality. CMCTS of silkworm pupa provided a moderate antioxidant activity and a significant inhibitory effect on the lipid peroxide level of human plasma at a concentration of more than or equal to 10 mM. These results proved that CMCTS of silkworm pupa was non-toxic substance also can be used as an alternative antioxidant source.

How to Cite this Article
Pubmed Style

Fadly D, Kusharto CM, Kustiyah L, Suptijah P, Muttalib YS, Bohari . In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. SRP. 2020; 11(7): 76-81. doi:10.31838/srp.2020.7.13

Web Style

Fadly D, Kusharto CM, Kustiyah L, Suptijah P, Muttalib YS, Bohari . In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. http://www.sysrevpharm.org/?mno=117583 [Access: March 29, 2021]. doi:10.31838/srp.2020.7.13

AMA (American Medical Association) Style

Fadly D, Kusharto CM, Kustiyah L, Suptijah P, Muttalib YS, Bohari . In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. SRP. 2020; 11(7): 76-81. doi:10.31838/srp.2020.7.13


Vancouver/ICMJE Style

Fadly D, Kusharto CM, Kustiyah L, Suptijah P, Muttalib YS, Bohari . In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. SRP. (2020), [cited March 29, 2021]; 11(7): 76-81. doi:10.31838/srp.2020.7.13


Harvard Style

Fadly, D., Kusharto, . C. M., Kustiyah, . L., Suptijah, . P., Muttalib, . Y. S. & Bohari, . (2020) In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. SRP, 11 (7), 76-81. doi:10.31838/srp.2020.7.13


Turabian Style

Fadly, Dzul, Clara M. Kusharto, Lilik Kustiyah, Pipih Suptijah, Yuges Saputri Muttalib, and Bohari. 2020. In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. Systematic Reviews in Pharmacy, 11 (7), 76-81. doi:10.31838/srp.2020.7.13


Chicago Style

Fadly, Dzul, Clara M. Kusharto, Lilik Kustiyah, Pipih Suptijah, Yuges Saputri Muttalib, and Bohari. "In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation." Systematic Reviews in Pharmacy 11 (2020), 76-81. doi:10.31838/srp.2020.7.13


MLA (The Modern Language Association) Style

Fadly, Dzul, Clara M. Kusharto, Lilik Kustiyah, Pipih Suptijah, Yuges Saputri Muttalib, and Bohari. "In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation." Systematic Reviews in Pharmacy 11.7 (2020), 76-81. Print. doi:10.31838/srp.2020.7.13


APA (American Psychological Association) Style

Fadly, D., Kusharto, . C. M., Kustiyah, . L., Suptijah, . P., Muttalib, . Y. S. & Bohari, . (2020) In Vitro Study of Antioxidant Activity of Carboxymethyl Chitosan derived from Silkworm (Bombyx mori L.) Pupa against Human Plasma Lipid Peroxidation. Systematic Reviews in Pharmacy, 11 (7), 76-81. doi:10.31838/srp.2020.7.13

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