Influence of Oocyte Collection Techniques on Oocyte Retrieval and Quality from Ovaries of Iraqi Goats (Capra Hircus)

Abstract

Ali J. AL-Nuaimi, Hashim M. AL-Rubaei and Fawzea G. AL-Gebouri

The objective of this current study to determine the influence of different three retrieval techniques which included slicing of ovary, puncture of ovary and aspiration of visible surface follicles on oocytes quantity and quality from goat ovaries. The ovaries were recovered from local slaughterhouse and oocyte was retrieval by using slicing, puncture and aspiration techniques. The harvested oocytes were classified according to cumulus cells surrounding them and homogenous of ooplasm into three grades, oocytes with at least three layers of cumulus cells oocytes with less than three layers of cumulus cells and denuded oocytes. The results showed that the average total number of oocytes retrieved per ovary was significantly increased (P<0.05) in slicing (6.8±0.11) than punctures (4.14±0.06) and aspiration (2.80±0.03) techniques. The rate of oocytes collected per ovary were 4.58±008 of which 2.01±0.12 (40.07%), 1.20±0.03 (28.93%) and 1.29±0.02(30.99%) were flood, fair and poor oocytes respectively. The average number and percentage of good grade oocytes were significantly increased (P<0.05) by slicing 3.56±0.04 (52.35%) than puncture 1.75±0.15 (42.55%) and the aspiration 0.72±0.08 (25.32%) techniques. Current results led to the conclusion that slicing technique was more efficient than puncture and aspiration techniques as oocytes retrieval technique on oocytes yielded and quality for goat oocytes.