Inhibitory Power Test of White Rice Bran Extract (Oryza sativa L.) with the Solution of Ethanol and Aquades on Porphyromonas gingivalis (In Vitro) Bacteria

Abstract

Harun Achmad, Marhamah F. Singgih, Huldani, Al Frida Ramdhani, Yunita Feby Ramadhany

Background: Bran is byproduct of the rice milling process and rice waste. Out of 32 million tons of rice obtained by rice bran as a byproduct of around 2,5 million tons. White rice bran (Oryza sativa L.) has many bioactive ingredients, one of which is a phenol compound. Phenol compound are known to have antibacterial activity. Porphyromonas gingivalis (p.gingivalis) is one of Gram negative anaerobic that contribute to the occurrence of periodontal disease, so that materials that have antibacterial properties are very necessary to be given to inhibit the growth of these bacteria. Objectives: Knowing the inhibitory effect of white rice bran extract (Oryza sativa L.) with ethanol and aquadest solvents to P.gingivalis bacteria at concentrations of 12,5%, 25%, 50%, 75% and to determine the difference in the effectiveness of white rice bran extract (Oryza sativa L.) with ethanol and aquadest solvents to P.gingivalis bacteria. Methods: This research was laboratory experiment with post test only control group design with diffusion method Kirby Bauer. In this research with 6 repetitions with extract at concentrations of 12,5%, 25%, 50%, 75%, positive control (Chlorhexidine), and negative control (aquadest). The measuring instrument in this research was using caliper with millimeters (mm). Analysis data was using Krusskal Wallis continued by Mann Whitney test. Results: The results showed that white rice bran extract at concentrations of 12,5%, 25%, 50%, 75% with ethanol and aquadest solvents are significantly influence (Krusskal Wallis test showed p value = 0,000 (p<0,005) to inhibit the growth of Porphyromonas gingivalis. Conclusions: white rice bran extract at concentrations of 12,5%, 25%, 50%, 75% with ethanol and aquadest solvents can inhibit the growth of Porphyromonas gingivalis bacteria and inhibit zone that ethanol result is more then aquadest solvent. The inhibition zone produced by white rice bran extract with ethanol solvent is almost the same as the chlorhexidine inhibition zone.