Isolation and Identification of Aspergillus spp. from Human and Sheep Respiratory Infection in Al-Qadisiyah Province

Abstract

Riyadh Ajmi Salman, L. Dr. Zainab A. A. Al-haddad

This study was conducted to isolate the most common Aspergillus spp. which cause dangerous diseases such as aspergillosis from respiratory infection in human and sheep, one hundred (100) human sputum sample collected from patients in respiratory diseases center of Al-diwaniyah educational hospital during a period of two (2) months wich where (November and December 2019), and one hundred (100) nasal swabs samples were collected from sheep which appeared clinical signs of respiratory infections from sheep flocks in rural in Al- Qadisiyah province, samples collected from sheep during the period of two months (2) which were January and february2020. The isolated species in human appeared 74 out of 100(74%) positive fungal isolate so Aspergillus fumigatus represents12 isolates (16.21 %), Aspergillus niger, 8 isolates ( 10.81%), Aspergillus flavus 5 isolates (6.75%), Aspergillus ochraceus 2 isolates (2.7%),also another spp. of fungi were isolated which include penicillium spp. and Alternaria spp. 7 isolates (9.45%) for each one of them, Geotrichum spp. 4 isolates (5.4%), Trichophyton mentagrophytes 3 isolates ( 4.05%), Cladosporium spp. 2 isolates (2.7%), whereas Yeasts spp. constitute 22 isolates (29.72%). While the sheep revealed ninety six (96) fungal isolate out of (100) which include: Aspergillus ochraceus 9 isolates (9.37%), Aspergillus niger 6 isolates (6.25%), Aspergillus flavus 3 isolates (3.12%), Aspergillus fumigatus 2 isolates (2.08%), also other fungal species were isolated include : Alternaria spp. 9 isolates(9.37%), Rhizopus spp. 14 isolates(14.58%), Trichophyton mentagrophytes 13isolates(13.54%), penicillium spp. 12isolates (12.5%), Trichophyton verrucosum10 isolates(10.41%), Microsporum caninum 6 isolates(6.25%) , Geotrichum spp. and Cladosporium spp. 1isolate(1.04%) for each one of them and Yeast spp. 10 isolates(10.41%).