Melatonin Levels in Shift Nurses with ELISA and LCMS Methods, Jakarta, Indonesia

Abstract

Yosephin Sri Sutanti, Muchtaruddin Mansyur, Nurhadi Ibrahim, Martina Wiwie S. Nasrun, Indah Suci Widyahening, Dewi Irawati, Yusra Yusra, Rimawati Tedjasukmana

A nurse has a high enough stress level because it is directly related to coping with various kinds of patients on shift work schedules. So far, the detection of stress cases is based on a questionnaire in which validity and relativity are quite good, although subjective, such as the Perceived Stress Score (PSS) questionnaire. This study aimed to examine objective markers of stress in shift working nurses by measuring melatonin levels. Methods: The study was conducted from December 2019 to June 2020. The respondents were the shift and non-shift nurses from one of the public hospitals in Jakarta, Indonesia, by obtained through by consecutive sampling. Each group consisted of 40 people each. Melatonin was measured twice, at midnight on the last night shift (second night) in the shift group (pre-work), then at 08.00 am on the following day (post-work). The non-shift group was carried out on weekdays at 08.00 am pre-work and at 04.00 pm post-work. Measurements used Enzyme-Linked Immunosorbent Assay (ELISA) and Liquid Chromatography-Mass Spectrometry (LCMS). Results: The percentage of nurses who showed moderate stress level in the shift group (30%) is higher than the non-shift (25%). There was a significant difference in the mean pre-work melatonin levels, measured with ELISA, between the shift and the non-shift group (51.53 pg/ml vs 17.07 pg/ml, P = ≤ 0,001), as well as the postwork examination (24.30 pg/ml vs 10.84 pg/ml, P = ≤ 0,001). We acquired a new reference for mean melatonin levels at 04.00 pm of 10.48 pg/mL (2.15-38.30 pg/mL) using ELISA and 38.13 pg/mL (5.00-230.00 pg/ mL) using LCMS in non-shift nurses. In addition, a new reference for melatonin levels at 12.00 pm was 51.53 pg/mL (0.80-135.00 pg/mL) using ELISA and 52.35 pg/ mL (10.00-170.00 pg/mL) using LCMS in shift nurses. Discussion: This study showed differences in the results of measuring melatonin levels using ELISA and LCMS, which will be a good starting point for further research with a larger number of respondents. In addition, it is interesting to observe that a decrease in the mean level of melatonin from pre-work to post-work was found in both the nightshift and dayshift groups, using ELISA and LCMS.

Most Viewed Articles
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli SRP. 2020; 11(1): 01-06 » doi: 10.5530/srp.2020.1.01
  • Psychometric properties of the World Health Organization Quality of life instrument, short form: Validity in the Vietnamese healthcare context Trung Quang Vo*, Bao Tran Thuy Tran, Ngan Thuy Nguyen, Tram ThiHuyen Nguyen, Thuy Phan Chung Tran SRP. 2020; 11(1): 14-22 » doi: 10.5530/srp.2019.1.3
  • A Review of Pharmacoeconomics: the key to “Healthcare for All” Hasamnis AA, Patil SS, Shaik Imam, Narendiran K SRP. 2019; 10(1): s40-s42 » doi: 10.5530/srp.2019.1s.21
  • Deuterium Depleted Water as an Adjuvant in Treatment of Cancer Anton Syroeshkin, Olga Levitskaya, Elena Uspenskaya, Tatiana Pleteneva, Daria Romaykina, Daria Ermakova SRP. 2019; 10(1): 112-117 » doi: 10.5530/srp.2019.1.19
Most Downloaded
  • Dental Development between Assisted Reproductive Therapy (Art) and Natural Conceived Children: A Comparative Pilot Study Norzaiti Mohd Kenali, Naimah Hasanah Mohd Fathil, Norbasyirah Bohari, Ahmad Faisal Ismail, Roszaman Ramli SRP. 2020; 11(1): 01-06 » doi: 10.5530/srp.2020.1.01
  • Manilkara zapota (L.) Royen Fruit Peel: A Phytochemical and Pharmacological Review Karle Pravin P, Dhawale Shashikant C SRP. 2019; 10(1): 11-14 » doi: 0.5530/srp.2019.1.2
  • Pharmacognostic and Phytopharmacological Overview on Bombax ceiba Pankaj Haribhau Chaudhary, Mukund Ganeshrao Tawar SRP. 2019; 10(1): 20-25 » doi: 10.5530/srp.2019.1.4
  • A Review of Pharmacoeconomics: the key to “Healthcare for All” Hasamnis AA, Patil SS, Shaik Imam, Narendiran K SRP. 2019; 10(1): s40-s42 » doi: 10.5530/srp.2019.1s.21
  • A Prospective Review on Phyto-Pharmacological Aspects of Andrographis paniculata Govindraj Akilandeswari, Arumugam Vijaya Anand, Palanisamy Sampathkumar, Puthamohan Vinayaga Moorthi, Basavaraju Preethi SRP. 2019; 10(1): 15-19 » doi: 10.5530/srp.2019.1.3
bandar togel terpercaya
agen togel terpercaya
situs togel bri online resmi terbaik dan terpercaya
aplikasi judi togel
daftar bandar lotre togel
bo togel deposit pulsa murah
Agen Togel Play Resmi
KEPO4D Toto Online Deposit Via Dana
bandar togel hadiah terbesar dan terpercaya DAPURTOTO
Daftar Togel Online Terbaik dan Terbesar
bandar togel terpercaya
agen togel online terpercaya
slot online gacor terbaik 2022 LAKU4D
bandar toto togel
Toto Togel laku4d
Situs Slot
Bandar Togel Online
Situs Bandar Togel
Bandar Togel 4D
Prediksi Togel Jitu
Situs Bandar Togel 4D
Bandar Togel 4D
Bandar Togel terlengkap
Bandar Togel online24jam
Bandar Togel hadiah 4d 10 juta
agen togel terpercaya
slot online dan judi bola dewa118
Situs Togel Online
Bandar Togel toto 4d terlengkap
data Sidney
toto slot
link alternatif situs togel
Situs Togel dan Toto Slot Online
bandar togel hadiah 4d 10 juta
toto hk
togel
data sdy
togel sgp
data togel sgp
bandar togel 4d dan slot deposit pulsa terpercaya
toto sgp
toto hk
togel hk
bandar togel
bandar togel 4d
togel 4d
situs togel deposit pulsa
toto online
BO Togel
BO Togel Toto HK
bandar togel 4d
totoslot 4d
situs togel resmi dan terpercaya
bandar togel bolak balik dibayar
agen togel online
Bandar Judi Mesin Slot Toto Togel 4D