Modified Protocol to Use Platelet Rich Fibrin (PRF) as a Local Antibiotic Delivery System: In Vitro study
Background: Plasma concentrate products have rapidly developed during the last few years, and it had significantly improved regenerative medicine because of its specific features which enhance healing and reduce pain after surgeries. This research aims to improve the antibacterial effectiveness of Platelet Rich Fibrin (PRF) by adding different forms of antibiotics and evaluate the physical and antibacterial properties of the new PRF.
Methods: In vitro experimental study was conducted; modified protocol was used which use two stages of centrifuging were used to prepare PRF. Bacterial colonies and Miller-Hinton medium was prepared. Two types of bacteria were used for the culturing and incubation (Staphylococcus aureus, Enterococcus faecalis). Different forms and volumes of antibacterial agents (Lincomycin) were incorporated with the PRF. Physical and antibacterial properties were evaluated.
Results: Ampoule and solution forms had the highest levels of bacterial growth inhibiting, and best physical properties, bacterial inhibiting lasted for 10 days in culture. Significant difference was noticed between the modified PRF and the control group (p<0.05). Adding (0.5 ml) of ampoule form had the best results on both antibacterial and physical properties of the new PRF.
Conclusion: The protocol proposed in this study succeeded to improve the antibacterial activity of the PRF with preserving the physical properties. No significant differences in physical properties were noticed between control group and PRF with antibiotic.
Significance and impact of the study: PRF can be used as a local antibiotic delivering biomaterial adjacent to minor surgery, the results of this study can be used to reduce the post-operative infection that is frequent after oral surgeries.