Molecular and susceptibility Study of Antibiotic Resistance Genes in E. coli Isolated from Selected Iraqi Patients

Abstract

Wedad Salih Dawood.

A number of mobile genetic elements, such as plasmids and transposons have recently been shown, to promote the exchange of genetic material between genera and bacterial organisms. The purpose of this research was to investigate the prevalence and diffusion of resistance genes in the sample community (n=100). It was found that at least one group of antibiotics had been resisted in 62% of the study population, a multi-resistance phenotype was seen in 22% and no resistance to any form of compounds was observed in 16%. In all isolates exhibiting Ampicillin resistance, the presence of �-lactamase TEM was observed. 12 of the isolates were identified for the gene encoding OXA. The existence of a CTX-M chloroidal lactamase was tested in 12 isolate compounds with the upstream insertion sequence ISEsp1. There are 17 of the isolates that have an int1 gene. This study concluded that ampicillin and cephalosporin, first generation, have demonstrated the greatest resistance to the use of these antibiotics as a single agent for the treatment of infections. The prevalence of different forms of osteoarthritis in the population of the sample, on the other hand, indicates the need for regular monitoring of the distribution of such determinants of resistance. The CTX-M-15 example of plasmid mediated �lactamases is alarming, given the simple discrimination between the same species or different species. The situation in these species is severe. The high incidence of resistant paediatric strain and multidrug-resistant strain in this same population is, after all, worried and needs close monitoring, because the care of selected patients is not recorded in the Baquba Teaching Hospital, Iraq, by many of the antimicrobials used.