Molecular detection of antibiotic resistance genes and biofilm production among uropathogenic bacteria

Abstract

Rawa Abdul Redha Aziz, Elaf Sameer.

Objective(s): UTI is showed to be the most popular infection among Iraqi community and nosocomial UTI and has high number of samples in clinical units and catheterized UTI patients. Aim of study is to detect the antibiotic resistant genes and biofilm production among Iraqi uropathogenic bacteria. Material and Methods: Fifty isolates of each Escherichia coli and Pseudomonas aeroginosa obtained from catheterized UTI patients admitted to Al- Najaf city hospitals. Vitek 2 automated system was used for identifying isolates. Antibiotic sensitivity was performed using disk diffusion method. PCR studies were done for detecting antibiotic resistance genes such as bla AMP, blaVIM, bla OXA58, rmtC, rmtF, rmt D, and parC. Microtiter plate assay was used to detect biofilm production ability. Results: Antibiotic sensitivity test revealed 60% of E. coli was MDR, 30% was XDR, 10% was PDR. P. aeroginosa showed 2% was pan drug resistant isolate, while there were 48% isolates considered as MDR and 50% were XDR. Antibiotic resistant genes were distributed among E. coli and P. aeroginosa isolates as follows: [50% and 68% for bla AMP], [90% and 55% for blaVIM], [80% and 50% for blaOXA58], [60% and 48% for rmtC], [58% and 40% for rmtF], [49% and 90% for rmtD], [80% and 78% for ParC], respectively. Microtiter dish assay demonstrated that 86% of E. coli and 90% of P. aeroginosa isolates were biofilm producers. Conclusion: Uropathogenic bacteria are being more antibiotic resistant by many means, causing serious problems for UTI catheterized patients in Al-Najaf city, Iraq.