Molecular Detection of Parvovirus B19 and Immunohistochemical Localization of Interleukin 6 of Tissues from Thyroid Cancer

Abstract

Jinan MJ Al-saffar, Saad Hasan Mohammed Ali, Shakir H. Mohammed Al-Alwany.

Background: Human parvovirus B19 has recently been detected in papillary thyroid carcinoma at a high frequency. An increased cytokines proinflammatory upregulation like interleukin-6 concentrations have been recently reported in the inflammation of thyroid tissues with possible role in thyroid cancer.
Objective: analyzing the compatible effect of IL-6 expression and Human parvovirus B19 infection on tissues out of several patients with benign and carcinomatous thyroid lesions and elucidate the associative role of Human B19 parvovirus in thyroid carcinogenesis.
Patients and methods: Ninety formelin-fixed, parraffin- embedded thyroid tissues registered within this study; 40 thyroid biopsies tissue out of papillary thyroid carcinoma (PTC) , 30 benign thyroid hyperplasia and 20 thyroid tissues with ordinary pathological variations, as an ostensibly tissues of healthy control. Detection of Human parvovirus B19 (hB19) was conducted through chromogenic in situ hybridization (CISH) method while the expressed protein of IL-6 gene through taking immunohisto-chemistry (IHC) system of recognition.
Results: In thyroid carcinoma, the Human parvovirus B19- positive CISH reactions and the IL-6- IHC positive reactions were investigated in 17.5% (7 out of 40) and 50% (20 out of 40) of tissues, respectively, while 6.7% (2 out of 30) and 26.7% (8 out of 30 ) of Human parvovirus B19- positivity and IL-6 -IHC reactions was detected in benign thyroid group. None of control thyroid tissues revealed CISH reaction while10% had positive IHC reaction. The correlation between Human parvovirus B19 and IL-6 was significantly important (P= 0.003).
Conclusions: The substantial IL-6 co-expression and Human parvovirus B19 could indicate their potential part played in either thyroid pathogenesis and / or carcinogenesis.