NEPHROPROTECTIVE EFFECTS OF QUERCETIN IN RENAL ISCHEMIA REPERFUSION INJURY IN MICE

Abstract

Widad Abd Al-Jabbar, Najah R Hadi, Fadhaa Abdul Ameer Ghafil, Hussein Abdulkadhim, Wadhah Mahbuba, Maysaa Ali Abdul Khaleq, Ghizal Fatima

The purpose of the present paper is to study the Nephroprotective l effect of Quercttin in renal ischemia reperfusion mice model. The methods are forty male Swiss Albino mice 7-15 weeks weighting 30- 35 gram (g) were divided into four groups, ten mice in each group, animal groups are arranged as, group I (Sham group) have been subjected to the same anesthetic and surgical laparotomy but without induction of IRI, Group II (Control group): Exposed to bilateral renal IR procedure under anesthesia, Group III (Vehicle group): Received vehicles sterile dimethyl sulfoxide (DMSO 0 .2 %) alone intraperitoneal 30 minutes before exposed to renal IR procedures under anesthesia, Group IV (Quercttin treated group ): Receiving Quercttin in a dose of 50 mg/kg intraperitoneal injection 30 minutes before exposed to IR procedure under anesthesia and served as treated group. Blood samples were collected directly from the carotid vein for determination of serum levels of urea & creatinine, NGAL and Notch level. Bilateral nephrectomy was done and homogenized for measurements of tissue markers (Interleukin6 (IL-6), Toll like receptors 2 (TLR-2), F2 Isoprostane, Bc12 associated X protein (Bax) and B cell lumphoma2 (Bcl2). In this in addition to mean serum levels of (Urea and Creatinine) and mean of plasma NGAL beside the mean of Notch1 Jagged 1 and scores for histopathological changes were significantly (*P<0.05) elevated in control group as compared with that of sham group while in Quercttin treated group the above mentioned parameters were significantly (*P<0.05) decreased, in addition to histopathological changes score and according to the mean tissue level of Bcl-2 the result was controversial. In conclusion, Quercttin possess a renoprotective effects via modulation of the inflammatory, oxidative and apoptotic pathway.