Nephroprotective Potential Effect of Quercttin in Renal Ischemia Reperfusion Injury in Rat Model (Role of Akt/m TOR Pathway)

Abstract

Rusul F. Hasan, Murooj L. Altimimi, Hayder M. Alaridy, Najah R. Hadi.

Ischemia reperfusion is determined by a limitation of blood supply to the organs and subsequent blood restoration and reoxygenation. Renal ischemia reperfusion injury (IRI) is a potential element in the pathogenesis of acute kidney injury arising from oxidative stress, inflammation and apoptosis of renal tubular cells. The mammalian target of rapamycin (mTOR) pathway activates after IRI and contributes to renal repair and regeneration. Quercetin is a flavonoid compound that presents in many fruits and vegetables. Quercetin is widely used for its anti-inflammatory and anti-oxidant effects. The present study aimed to investigate the potential protective effect of quercetin on renal IRI in rat model by regulation of Akt/mTOR pathway. At the end of experiment, the serum levels of urea and creatinine and renal tissue levels regarding p-mTOR, p-Akt, IL-1β, caspase-3, and F2-isoprostane were recognized to be significantly (p value<0.05) elevated in control group when compared with sham group. Histopathologic alanalysis of the renal tissues recognized a severe renal injury in control group and there was a significant role of quercetin in ameliorating this injury by minimizing the serum levels of urea and creatinine, tissue levels of IL-1β, caspase-3, F2-isoprostane except for p-mTOR and p-AKt levels which were significantly (p value<0.05) higher in comparison with that of control group. In addition, treatment of quercetin significantly (p<0.05) reversed the histopathologic severity scores of renal tissue damage. From the overall results, we concluded that quercetin significantly diminished renal ischemia reperfusion injury in the rat model through Akt/mTOR pathway by its effect as anti-oxidant, anti-inflammatory and anti-apoptotic.