Next-Generation Sequencing Technologies for Environmental DNA as an Efficient Bio Indicator for Bacterial Biodiversity in Tigris River, Iraq

Abstract

Fikrat M Hassan, Warqaa Y. Salih and Halah H Al-Haideri

Access to multiple, large sequence data volumes, as well as further improvements in read length and sequence platform efficiency, lead to better sample diversity. In order to assess the ability of Next Generation Sequence (NGS) approach to establish and evaluate the environmental molecular diagnostics, directly monitor bulk bacterial communities, the Illumina system of bacterial 16S rRNA gene amplicon was investigated in total bacterial communities in water samples. The dominant bacterial species can be identified through this method. NGS data will also allow the identification of drivers of the composition of bacterial communities during water treatment and distribution in combination with metadata, demonstrating the power of this approach to track bacterial regrowth and contamination in technical systems.