Osmotic Fragility And Hemolysis% Of Human Erythrocytes �?-Thalassemia Major And Anemia Patients Compared To Healthy Subjects

Abstract

Aseel Sh. Abdullah, Yamama Zuhair, Alaa Anwer, Israa Ghassan Zainal.

Contemplating the osmotic conduct and hemolysis% of human erythrocytes isn't just significant clinically, yet it is additionally considerable in comprehension of material transfer across biological membranes. The fragile gram piece of the spot of hemolysis were viewed as an element of extra-cellular osmolarity. The object of this field is to assess the (hemolysis% and osmotic fragility) tests of "β – thalassemia major and anemia" patients erythrocytes compared with healthy subjects and to assess the chance of utilizing any of them as a fundamental screening instrument for "β – thalassemia major and frailty" patients. Hemolysis % and osmotic fragility tests were examined for (40 β – thalassemia major and 25 anemia) patients contrasted with 20 healthy subjects. There was an expansion in the hemolysis % for "β-thalassemia major and anemia" patients by (176 and 273.5) % individually contrasted with the typical erythrocytes. The fragility curve by utilizing NaCl on "β-thalassemia and anemia" patients erythrocytes demonstrated move towards lower NaCl concentration showing that the normal osmotic fragility H50 (the NaCl fixation delivering half homolysis) to be (0.1 and 0.2)% of "β – thalassemia major and anemia" patients Erythrocytes separately contrasted with (0.45 %) healthy subjects. In addition to use different concentrations from saline (NaCl) solution, this study tested the osmotic behavior of human RBCs for all studied groups using H2O2 as another osmolytes. The data are clearly different from to that spotted with sodium chloride. Fragility grams of human RBCs showed that with increase the concentration of H2O2 the H% were increased. Finally, H50 equal to 0.5%, 0.45% for "β – thalassemia major and anemia" patients and 0.5% of healthy subjects.